文献类型：期刊文献

中文题名：四神丸对脾肾阳虚型溃疡性结肠炎大鼠血清TGFβ1、IL-6及结肠组织TLR-4 mRNA表达的影响

英文题名：Effect of Sishen Pill on Serum of TGFβ1,IL-6 and Colonic Tissue of TLR-4 mRNA Expression in Rats with Ulcerative Colitis of Spleen-kidney Deficiency Type

作者：王燕[1];何兰娟[1];朱向东[1];郭婷婷[1];王迪[1]

第一作者：王燕

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：43

期号：5

起止页码：118

中文期刊名：中医药学报

外文期刊名：Acta Chinese Medicine and Pharmacology

收录：CSTPCD

基金：国家自然科学基金项目(81360541)

语种：中文

中文关键词：溃疡性结肠炎;四神丸;转化生长因子β1;白介素6;Toll样受体4

外文关键词：Ulcerative colitis（UC）; Sishen pill; Transforming growth factor beta1（TGF β1）; Interleukin 6（IL-6）; Toll-like receptor 4（TLR-4）

摘要：目的:观察四神丸对脾肾阳虚型溃疡性结肠炎大鼠中血清转化生长因子β1(TGFβ1)、白介素6(IL-6)及结肠组织中Toll样受体4(TLR-4)mRNA含量的变化,探讨四神丸对脾肾阳虚型溃疡性结肠炎的作用机制。方法:采用番泻叶灌胃+氢化可的松注射液腹腔注射+2、4、6-三硝基苯磺酸(TNBS)/乙醇法灌肠建立脾肾阳虚型溃疡性结肠炎大鼠模型。将60只wistar大鼠随机分为空白组、模型组、四神丸组及柳氮磺嘧啶(SASP)组,经药物干预后分别采用酶联免疫法检测各组大鼠血清中的TGFβ1、IL-6含量及半定量反转录-聚合酶链反应检测各组大鼠结肠组织中的TLR-4 mRNA含量。结果:与空白组比较,模型组大鼠中TGFβ1含量降低(P<0.05),IL-6含量升高(P<0.05);与模型组比较,各治疗组大鼠中TGFβ1含量升高(P<0.05),IL-6含量降低(P<0.05)。与空白组比较,模型组大鼠结肠组织TLR-4 mRNA表达显著增强(P<0.05);各治疗组TLR-4 mRNA表达减弱(P<0.05)。结论:四神丸能够通过下调IL-6和升高TGFβ1的表达,降低结肠组织中TLR-4 mRNA的含量,抑制炎症反应,调节肠上皮免疫系统,恢复肠粘膜微环境的免疫稳态,达到治疗UC的目的。
Objective： To observe the influence of Sishen pill on serum of transforming growth factor beta 1（ TGF β1）,interleukin 6（ IL-6） and colonic tissue of toll-like receptor 4（ TLR-4） mRNA content changes and identify its underlying mechanism of action. Methods： Use lavage of senna ＋ intraperitoneal injection of hydrocortisone injection ＋ enema of 2,4,6-trinitrobenzene sulfonic acid（ TNBS） / ethanol to establish the rat models of ulcerative colitis with spleen-kidney deficiency type. 60 wistar rats were randomly divided into a blank group,a model group,a Sishen pill group and a salazosulfadimidine（ SASP） group. After drug intervention,serums of TGF β1 and IL-6 were detected by ELISA,and the colonic tissue of TNF-αmRNA was detected by Sq RT-PCR. Results： Compared with those in the blank group,in the model group rats＇ TGFβ1 was decreased（ P〈0. 05）,but IL-6 content was increased（ P〈0. 05）; compared with those in the model group,in the treatment group TGF β1 content was increased（ P〈0. 05）,but IL-6 was decreased（ P〈0. 05）. Compared with that in the blank group,the expression of the model group＇s TLR-4 mRNA of colonic tissue was significantly enhanced（ P〈0. 05）; the expression of each treatment group＇s TLR-4 mRNA was decreased（ P〈0. 05）. Conclusion： Sishen pill can decrease the expression of IL-6,increase the expression of TGF β1,reduce content of TLR-4 mRNA in colonic tissue,and then it can inhibit the reaction of inflammation,regulate intestinal epithelial immune system,restore intestinal mucosal immune steady-state microenvironment,then achieve the purpose of the treatment of UC.