文献类型：期刊文献

中文题名：芍药苷对溃疡性结肠炎大鼠结肠组织病理形态及血清抗炎和促炎因子平衡的影响

英文题名：Effect of paeoniflorin on colon tissue pathological morphology and the balance of anti-inflammatory and proinflammatory factors in rats with ulcerative colitis

作者：汪斌[1];朱向东[1,2];段永强[1,3];李兰珍[1];曹燕飞[1]

第一作者：汪斌

机构：[1]甘肃中医学院中医基础理论教研室,甘肃兰州730000;[2]南京中医药大学基础医学院,江苏南京210000;[3]兰州大学医学院,甘肃兰州73000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2013

卷号：21

期号：1

起止页码：1

中文期刊名：中国中西医结合消化杂志

外文期刊名：Chinese Journal of Integrated Traditional and Western Medicine on Digestion

收录：CSTPCD

基金：国家自然科学基金(No:81060283);甘肃省自然科学基金(No:1107RJZA222)

语种：中文

中文关键词：结肠炎,溃疡性;芍药苷;抗炎因子、抑炎因子

外文关键词：ulcerative colitis; paeoniflorin; anti-inflammatory factor; inhibit inflammation factor

摘要：[目的]观察芍药苷治疗溃疡性结肠炎(ulcerative colitis,UC)的疗效,并探讨其作用机制。[方法]40只大鼠中10只为空白组,30只采用TNBS/乙醇灌肠法制备UC大鼠模型后分为模型组、芍药苷组及SASP组,光镜观察各组结肠组织病理形态变化并评分,采用ELISA法检测各组大鼠血清白细胞介素(IL)-2、IL-10、IL-6水平。[结果]与空白组大鼠比较,模型组大鼠血清IL-2[(43.05±8.42)ng/L]、IL-6[(248.05±46.56)ng/L]含量均明显升高,IL-10[(23.47±7.66)ng/L]含量显著下降,差异具有统计学意义(P<0.01);芍药苷组血清IL-2[(28.25±1.25)ng/L]、IL-6[(186.56±47.14)ng/L]水平均较模型组降低,而IL-10[(42.29±5.11)ng/L]水平明显升高,差异具有统计学意义(P<0.05、P<0.01)。[结论]芍药苷能够通过下调促炎因子IL-2、IL-6的表达,升高抗炎因子IL-10的表达,促进抗炎和抑炎因子的平衡,调节肠道异常免疫反应、抑制炎症反应,从而修复溃疡。
[Objective]To observe the effect of paeoniflorin on ulcerative colitis （UC） and to investigate the possible mechanism. [Methods]The UC rat models were prepared by the TNBS/ethanol enema, then randomly divided into three groups of model group,paeoniflorin group and SASP group （each n= 10） and 10 rats as normal control. The colon tissue pathological morphology was observed and scored under the light microscope,and the levels of IL-2, IL-10 and IL-6 were detected in rat serum. [Results]Compared with the control group, the content of IL-2 （[43.05 ± 8. 42] ng/L）, IL-6 （[248.05± 46.56] ng/L） was markedly higher, and that of IL-10（[23.47±7.66] ng/L）was decreased significantly in rats of the model group （P〈0. 01）. The content of IL-2（[28. 25-±t-1.25] ng/L）and IL-6（[186.56_±47.14] ng/L） in rats of the paeoniflorin groups were lower, and that of IL-10（42.29±5. 111 ng/L） was higher than those of the model group respectively （P〈0.05, P〈0.01）. [Conelusion]The paeoniflorin can decrease the expression of IL-2, IL-6,and increase the expression of IL-10, which might be one of the mechanisms of inhibition of inflammation for UC.