文献类型：期刊文献

英文题名：Optimized extraction and purification of geniposidic acid from Eucommia ulmoides leaves and its Anti-Parkinson's disease activity

作者：Zhang, Jiaxu[1];Dong, Xiaoli[1];Li, Jiaojiao[1];Ma, Quhuan[2];Xie, Xingwen[3];Shi, Xiaofeng[2]

第一作者：张菊秀;张建勋;张静霞

通信作者：Shi, XF[1];Xie, XW[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Acad Inst Med Res, Inst Pharmaceut Res, 2 Xiaoxihu East St, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Clin Coll Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Prov Acad Inst Med Res, Inst Pharmaceut Res, 2 Xiaoxihu East St, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Clin Coll Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：238

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20254719541216);Scopus(收录号：2-s2.0-105022081817);WOS:【SCI-EXPANDED(收录号:WOS:001625232700001)】；

基金：This research was funded by Gansu Provincial Traditional Chinese Medicine Industry Innovation Consortium Project (22ZD6FA021-4) .

语种：英文

外文关键词：Eucommia ulmoides leaves; Geniposidic acid; Response surface methodology; Macroporous adsorbent resin; Caenorhabditis elegans; Parkinson's disease

摘要：This study presents a scalable and efficient approach for extracting the bioactive iridoid compound geniposidic acid (GPA) from Eucommia ulmoides leaves (E. ulmoides leaves) by optimizing an ultrasound-assisted extraction process using response surface methodology (RSM). Under the optimized conditions-which include a 49 % ethanol volume fraction, a solid-to-liquid ratio of 1:25 g/mL, a 60 min extraction time, and a temperature of 60 degrees C-the GPA yield reached 10.53 +/- 0.14 mg/g. Following this, a systematic evaluation of macroporous resins based on adsorption and desorption performance identified H103 resin as the most suitable purification medium. Using this resin, the optimized chromatographic process achieved an adsorption efficiency of 96 %. After impurities were removed with 4 bed volumes (BV) of purified water, gradient elution with 6 BV of 5 % ethanol produced a GPA-enriched fraction with a purity of 16.53 +/- 0.43 %, representing a 6.43-fold increase compared to the initial extract. Further purification via preparative high-performance liquid chromatography (Prep-HPLC) yielded a GPA monomer with over 95 % purity. To validate its biological activity, in vivo testing was conducted using a Caenorhabditis elegans (C. elegans) model of Parkinson's disease (PD). The results showed that high-purity GPA significantly enhanced the health status of C. elegans, effectively suppressed the abnormal aggregation of alpha-synuclein (alpha-syn), and mitigated both acute and chronic dopaminergic neuronal damage. This study provides a robust scientific foundation for the large-scale production of GPA and highlights its promising neuroprotective potential.