文献类型：期刊文献

中文题名：枸杞多糖对慢性心理应激大鼠空间学习记忆能力的影响

英文题名：Effects of lycium barbarum polysaccharide on spatial learning and memory in rats with chronic psychological stress

作者：余娟[1];潘桃[2]

第一作者：余娟

机构：[1]甘肃中医药大学心理学教研室,甘肃兰州730000;[2]重庆市璧山区人民医院急诊科,重庆402760

第一机构：甘肃中医药大学人文与外国语学院（甘肃中医药文化传承发展中心办公室）

年份：2020

卷号：36

期号：15

起止页码：2290

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

语种：中文

中文关键词：慢性心理应激;枸杞多糖;空间学习记忆;下丘脑-垂体-肾上腺轴

外文关键词：chronic psychological stress;lycium barbarum polysaccharide;spatial learning and memory;hypothalamic-pituitary-adrenal axis

摘要：目的研究枸杞多糖(LBP)对慢性心理应激大鼠空间学习记忆能力的影响及其相关机制。方法SD大鼠90只,随机分为对照组(n=30)、模型组(n=30)及实验组(n=30)。模型组及实验组大鼠均采用束缚制动法制备慢性心理应激大鼠模型,造模成功后,实验组大鼠给予LBP 250 mg·kg^-1·d^-1,灌胃,对照组与模型组则灌胃等量生理盐水,连续干预6周。用Morris水迷宫测试仪检测大鼠空间学习记忆能力;用酶联免疫吸附(ELISA)法检测血清促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)和皮质酮(CORT)水平;用免疫组化法及逆转录-聚合酶链反应法检测海马组织糖皮质激素受体(GR)蛋白及mRNA表达。结果对照组、模型组及实验组大鼠逃逸潜伏期分别为(15.64±1.25),(40.23±20.11)和(21.45±9.84)s;穿越原平台次数分别为(6.33±2.14),(1.92±1.21)和(4.74±2.56)次;目标象限滞留时间百分比分别为(42.51±11.39)%,(18.61±4.92)%和(33.57±11.63)%;各组大鼠血清CRH水平分别为(0.68±0.03),(1.91±1.02)和(1.36±0.09)ng·mL^-1;ACTH水平分别为(26.42±5.74),(85.78±11.43)和(42.11±8.52)ng·L^-1;CORT水平分别为(4.64±1.31),(16.56±5.23)和(8.47±2.35)ng·mL^-1。与对照组相比,模型组和实验组大鼠逃逸潜伏期显著延长,且实验组短于模型组;穿越原平台次数及目标象限滞留时间百分比减少,且实验组多于模型组;血清CRH、ACTH及CORT水平升高,且实验组低于模型组;GR蛋白阳性细胞百分比与mRNA相对表达量降低,且实验组高于模型组(均P<0.05)。结论LBP可改善慢性心理应激大鼠的空间学习记忆能力,其作用机制可能与抑制下丘脑-垂体-肾上腺轴(HPA)的过度激活有关。
Objective To investigate the effects of lycium barbarum polysaccharide(LBP)on spatial learning and memory in rats with chronic psychological stress and its related mechanisms.Methods Ninety SD rats were randomly divided into control group(n=30),model group(n=30)and test group(n=30).Rats in model group and test group were established the chronic psychological stress rat models by the restraint braking method.After successful modeling,rats in test group were gavaged with LBP 250 mg·kg^-1·d^-1,control group and model group were gavaged with the same amount of normal saline for 6 weeks.The spatial learning and memory ability of rats were detected by Morris water maze;serum corticotropin releasing hormone(CRH),adrenocorticotropic hormone(ACTH)and corticosterone(CORT)levels were measured by enzyme-linked immunosorbent assay(ELISA)method;the expression of glucocorticoid receptor(GR)protein and mRNA in hippocampus was detected by immunohistochemistry method and reverse transcription-polymerase chain reaction method.Results The escape latency of control group,model group and test group were(15.64±1.25),(40.23±20.11)and(21.45±9.84)s;the number of crossing the original platform were(6.33±2.14),(1.92±1.21)and(4.74±2.56)times;the percentage of target quadrant retention time were(42.51±11.39)%,(18.61±4.92)%,(33.57±11.63)%;the serum CRH levels were(0.68±0.03),(1.91±1.02),and(1.36±0.09)ng·mL^-1;ACTH levels were(26.42±5.74),(85.78±11.43),(42.11±8.52)ng·L^-1;CORT levels were(4.64±1.31),(16.56±5.23)and(8.47±2.35)ng·mL^-1.Compared with control group,the escape latency of model group and test group were significantly prolonged,and test group was shorter than model group.The number of times of crossing the original platform and the percentage of retention time of the target quadrant were decreased,and test group was more than model group.Serum CRH,ACTH and CORT levels were significantly increased,and test group was lower than model group;the percentage of GR protein positive cells and mRNA relative expression were decreased,and test group was higher than model group(all P<0.05).Conclusion LBP can improve the spatial learning and memory ability of rats with chronic psychological stress,and the mechanism may be related to the inhibition of excessive activation of the hypothalamic-pituitary-adrenal(HPA)axis.