文献类型：期刊文献

中文题名：参桂保心方对心力衰竭大鼠Caspase-3及心室重构的影响

英文题名：Effect of Shengui Baoxin prescription on caspase-3 and ventricular remodeling in rats with heart failure

作者：卢玉俊[1];杨建芳[2];赵信科[1];代晶晶[1]

第一作者：卢玉俊

机构：[1]甘肃中医药大学附属医院,甘肃兰州730020;[2]甘肃省第三人民医院,甘肃兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2018

卷号：34

期号：5

起止页码：168

中文期刊名：湖南中医杂志

外文期刊名：Hunan Journal of Traditional Chinese Medicine

基金：甘肃省自然科学基金资助项目(编号:148RJZA068)

语种：中文

中文关键词：心力衰竭;Caspase-3;心室重构;参桂保心方;实验研究

外文关键词：heart failure;caspase - 3;ventricular remodeling;Shengui Baoxin prescription;experimental study

摘要：目的:通过观察参桂保心方对慢性心力衰竭(CHF)大鼠Caspase-3蛋白表达及其对心电图、血流动力学及心室重量指数等的影响,探讨该药保护心肌细胞、抑制心室重构的作用及其机制。方法:将90只雄性SD大鼠随机分为对照组15只和造模组75只,造模组腹腔注射ISO 3mg/(kg·d)制备CHF模型,4周后心脏彩超检测射血分数≤50%为造模成功。将其随机分为模型组,芪苈强心组,参桂保心高、中、低剂量组,每组15只,灌胃给药8周后记录各组大鼠心电图,监测血流动力学指标,测定心脏及心室重量指数,采用免疫组化、蛋白印迹法检测心肌Caspase-3蛋白表达。结果:治疗后参桂保心方高剂量组HR低于中、低剂量组及芪苈强心组(P<0.05);各组HWI、VWI均下降(P<0.05);芪苈强心组和参桂保心方高、中剂量组Caspase-3蛋白表达均降低,其中高剂量组优于其余2组(均P<0.05)。结论:参桂保心方可能通过减少Caspase-3蛋白表达,抑制心肌细胞凋亡,达到保护心肌细胞、抑制心室重构的作用。
Objective： To investigate the effect of Shengui Baoxin prescription on the protein expression of caspase-3,electrocardiographic findings,hemodynamic parameters,and ventricular weight index in rats with chronic heart failure（ CHF）,as well as the role of Shengui Baoxin prescription in protecting cardiomyocytes and inhibiting ventricular remodeling and the related mechanism. Methods： A total of 90 male Sprague-Dawley rats were randomly divided into control group with 15 rats and modeling group with 75 rats. The rats in the modeling group were given intraperitoneally injected ISO 3 mg/kg/d to establish a model of CHF,and the model was considered to be successfully established when echocardiography performed 4 weeks later showed an ejection fraction of ≤50%. The rats in the modeling group were further randomly divided into model group,Qili Qiangxin group,and low-,middle-,and high-dose Shengui Baoxin groups,with 15 rats in each group. After the drugs were given by gavage for 8 weeks,electrocardiographic findings were recorded,hemodynamic parameters were monitored,and the heart weight index and ventricular weight index were measured. Immunohistochemistry and Western blot were used to measure the expression of caspase-3 in the myocardium. Results： After treatment,the high-dose Shengui Baoxin group had a significantly lower heart rate than the middle-and low-dose Shengui Baoxin groups and the Qili Qiangxin group（ P〈0. 05）. All groups had significant reductions in the heart weight index and ventricular weight index（ P〈0. 05）. The Qili Qiangxin group and the high-and middle-dose Shengui Baoxin groups had a significant reduction in the expression of caspase-3（ P〈0. 05）,and the high-dose Shengui Baoxin group had a significantly greater reduction than the other two groups（ P〈0. 05）. Conclusion： Shengui Baoxin prescription can protect the cardiomyocytes and inhibit ventricular remodeling by reducing the expression of caspase-3 and inhibiting cardiomyocyte apoptosis.