文献类型：期刊文献

中文题名：甘肃野生秦艽rDNA ITS区序列分析

英文题名：rDNA ITS sequence analysis of wild Gentiana from Gansu province

作者：刘丽莎[1];王香梅[1];王昕[1];王岚[1]

第一作者：刘丽莎

机构：[1]甘肃中医学院基础课部,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2010

卷号：35

期号：5

起止页码：565

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：MEDLINE(收录号：20506812);CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家中医药管理局项目(04-C52L23);甘肃自然科学基金项目(3ZS041-A25-066)

语种：中文

中文关键词：甘肃;秦艽;ITS序列

外文关键词：Gansu province ; Gentiana ; ITS sequence

摘要：目的:比较研究甘肃不同地区4种野生秦艽rDNA ITS碱基序列的差异及其规律,寻找秦艽组药材之间的分子鉴别方法。方法:对rDNA ITS区进行了PCR扩增、测序,运用Clustal X,MEGA3等软件对ITS区序列进行分析。结果:不同地区秦艽、麻花秦艽、小秦艽及黄管秦艽的ITS长度为800 bp,ITS1,5.8S和ITS2序列长度分别为290,170,340 bp。根据两者序列以邻接法建立分子系统发生树。结论:ITS序列可以作为野生秦艽分子鉴定的依据。
Objective： To find the patterns of the rDNA ITS sequence variation in Gentiana, and establish the molecular biological method for the identification of the four kinds wild Gentiana from different regions in Gansu. Method： The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of Clustal X, MEGA3. Result： The Complete ITS sequence of G. macrophylla, G. straminea, G. dahurica and G. officinale was 800 bp, The sequences of ITS1, ITS2 and 5.8S were 290,340,170 bp, respectively. Phylogenetic tree based on ITS1 and ITS2 sequences data was constrcuted by Neighbor-joining method. Conclusion： ITS sequence could be as the evidence for the molecular authentication of Gentiana.