文献类型：期刊文献

中文题名：RP-HPLC法测定展筋酊中大黄素、大黄酚的含量

英文题名：Content Determination of Emodin and Chrysophanol in ZhanJing Tincture by RP-HPLC

作者：郭钰龙[1];王谨慧[2];靳子明[2];陶翠祥[2];杨锡仓[2]

第一作者：郭钰龙

机构：[1]甘肃中医学院,甘肃兰州730020;[2]甘肃中医学院附属医院

第一机构：甘肃中医药大学

年份：2013

卷号：26

期号：5

起止页码：24

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：甘肃省科学事业费资助项目(编号GS061-C33-29)

语种：中文

中文关键词：展筋酊;HPLC法;大黄素;大黄酚;含量测定

外文关键词：ZhanJing tincture; HPLC method; emodin; chrysophanol; content determination

摘要：目的:建立展筋酊中大黄素,大黄酚的RP-HPLC测定方法。方法:Symmetry C18色谱柱(5.0μm),甲醇∶0.1%磷酸溶液(85∶15)为流动相,流速:0.8 mL/min;柱温:20℃,检测波长:423 nm。结果:大黄素、大黄酚在10.0～30.0μg/mL浓度范围内与峰面积呈良好的线形关系(r=0.999 9,0.999 7);平均回收率分别为101.35%和98.25%(RSD=2.26%,1.17%)。结论:本方法快速、灵敏、专属性强,可用于展筋酊的含量测定。
Objective： To establish a RP-HPLC method for the determination ofemodin and ehrysophanol in ZhanJing tincture. Method： Symmetry C18 chromatographic column （5.0 μm） was adopted, methanol-0.1% phospho- ric acid （85 ： 1 5）as the mobile phase, flow rate was 0.8 ml. min-1; the column temperature was 20℃, the detection wavelength was 423nm. Result： Emodin and chrysophanol showed better linear relationship in the concentration range of 10.0 to 30.0 μg/mL and peak area （r=0.999 9, 0.999 7）; average recovery rates were 101.35% and 98.25% respectively （RSD=2.26%, 1.17%）. Conclusion： The method, rapid, sensitive and specific, could be used for the con- tent determination of ZhanJing tincture.