文献类型：期刊文献

中文题名：温通针法对血管性痴呆大鼠脑组织SOD、MDA及AChE的影响

英文题名：Effect of Warmth-promotion Needling on Cerebral SOD,MDA and AChE in Vascular Dementia Rats

作者：杨晓波[1];口锁堂[2];郑魁山[1]

第一作者：杨晓波

机构：[1]甘肃中医学院,兰州730000;[2]上海中医药大学,上海200032

第一机构：甘肃中医药大学

年份：2007

卷号：32

期号：3

起止页码：170

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：MEDLINE(收录号：17691574);CSTPCD;;Scopus(收录号：2-s2.0-34548082838);CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：血管性痴呆;温通针法;超氧化物歧化酶;丙二醛;乙酰胆碱酯酶

外文关键词：Vascular dementia; Warmth-promotion needling; Cerebral SOD, MDA and AChE

摘要：目的:观察温通针法对血管性痴呆(VD)模型大鼠脑组织内超氧化物歧化酶(SOD)、丙二醛(MDA)及乙酰胆碱酯酶(AChE)的影响,并探讨其相关机制。方法:将50只健康Wistar大鼠随机分为正常组、模型组、药物组、捻转针法组和温通针法组,采用反复夹闭双侧颈总动脉-再灌注方法造成血管性痴呆大鼠模型。针刺选穴为“大椎”“百会”“水沟”,分别施以温通或捻转手法;药物使用尼莫地平,0.0108 g/kg灌胃给药。治疗均每日1次,连续15 d。用比色法检测大鼠脑内MDA含量及SOD、AChE活性。结果:血管性痴呆模型组大鼠SOD活性较正常组显著降低(P<0.01),AChE活性、MDA含量较正常组皆显著升高(P<0.01);温通针法组、捻转针法组和药物组分别与模型组比较,均能明显升高SOD活性(P<0.01),降低AChE活性、MDA含量(P<0.05);温通针法组与药物组相比,升高SOD和降低AChE活性无显著性差异(P>0.05),降低MDA含量有显著差异(P<0.05);且温通针法组升高SOD活性,降低AChE活性、MDA含量的作用优于捻转针法组(P<0.05)。结论:温通针法具有抗脂质过氧化损伤及改善脑内胆碱能系统的能力。
Objective： To observe the effect of warmth-promotion needling on cerebral malondialdehyde （MDA） content, superoxide dismutase （SOD） and acetylcholinesterase （ACHE） activity so as to explore its underlying mechanism in improving vascular dementia （VD）. Methods： Forty Wistar rats were randomized into control, model, medication, needle-twirling and warmth-promotion groups with 8 cases in each. VD model was established by occlusion of the bilateral common carotid arteries and reperfusion in combination with intraperitoneal injection of sodium nitroprusside （2.5 mg/kg）. Warmth-promotion needling was applied to ＂Dazhui＂ （GV 14）, ＂Baihui＂ （GV 20） and ＂Shuigou＂ （GV 26）. Rats of medication group were intragastric perfusion of Nimodipine suspension （0. 0108 g/kg）. The treatment was given once daily continuously for 15 days. SOD and AChE activity and ME）A content of the brain （right side） tissue were detected with purine oxidase method, hydroxytamine chromatometry and thio-malonylurea method separately. Results： In comparison with control group, SOD activity of model group decreased significantly （P〈0.01 ）, AChE activity and MDA content increased considerably （P〈0.01）; while compared with model group, SOD activity of warmth-promotion, needle-twirling and medication groups raised evidently （P〈0.01 ）, AChE activity and MDA content of the later 3 groups lowered significantly （P〈0.05,0.01 ）. The effects of warmth-promotion group were significantly superior to that of medication group in lowering MDA content and to those of needle-twirling group in raising SOD activity and lowering AChE activity and MDA content （P〈0.05）. Conclusion： Warmth-promotion needling can function well in resisting lipid peroxidation injury and lowering AChE activity in VD rats, which may contribute to its effect in improving learning-memory ability.