文献类型：期刊文献

中文题名：组织培养技术及其在脱毒马铃薯种薯生产中的应用

英文题名：The tissue culture technology and its application in the production of the Virus-free potato seeds

作者：韩黎明[1]

第一作者：韩黎明

机构：[1]定西师范高等专科学校生物化学系,甘肃定西743000

第一机构：甘肃中医药大学定西校区

年份：2010

卷号：20

期号：1

起止页码：118

中文期刊名：信阳农业高等专科学校学报

外文期刊名：Journal of Xinyang Agricultural College

语种：中文

中文关键词：马铃薯;茎尖培养;脱毒苗;繁育

外文关键词：potatoes; stem tip culturing; virus-free seedling; proliferation

摘要：马铃薯退化是由于无性繁殖导致病毒连年积累而形成,而马铃薯幼苗的生长点的生长速度大于病毒繁殖速度,利用这个生长时间差切取茎尖进行组织培养可获得脱毒苗,由脱毒苗快速繁殖可获得脱毒种薯。制取马铃薯脱毒苗的关键技术是茎尖培养、病毒检测、脱毒苗快繁。
Potato degradation results from the accumulating of viruses caused by the asexual reproduction for successive years. The seedlings of the potatoes grow much quicker than the viruses can multiply during the same period. By using the time gap between them, the stem tip can be dissected and cultivated. In so doing, the virus-free potato seedling can be obtained. The quick multiplication of the seedling leads to virus-free potato seeds. The key technologies in obtaining the virus-free seedling are the stem tip culturing, virus checking, and the proliferating of the virus-free seedling.