文献类型：期刊文献

中文题名：当归不同有效部位对高原低氧模型小鼠免疫功能的影响

英文题名：Effects of Active Fraction of Angelica Sinensis Radix on Immunological Function in Mice under High Altitude Hypoxia Condition

作者：安方玉[1];刘永琦[1,2];骆亚莉[1];李应东[3];刘雪松[1];张齐[2];蔡路路[4];孙丽姣[4]

第一作者：安方玉

机构：[1]甘肃中医学院系统生物学与中医药转化研究所,甘肃兰州730000;[2]甘肃中医学院中药药理与毒理学省重点实验室,甘肃兰州730000;[3]甘肃中医学院附属医院,甘肃兰州730000;[4]甘肃中医学院公共卫生学院,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2015

卷号：22

期号：2

起止页码：51

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：国家科技支撑计划(2011BAI05B02)

语种：中文

中文关键词：当归有效部位;高原低氧;免疫功能;白细胞介素-2;小鼠

外文关键词：active fraction of Angelica Sinensis Radix;;high altitude hypoxia;;immunological function;;IL-2;;mice

摘要：目的探讨当归不同有效部位对高原低氧模型小鼠免疫功能的干预作用。方法将SPF级小鼠采用随机数字表法分为对照组、模型组、红景天组及当归有效部位B、X、C组。灌胃给药,每日1次,连续21 d。除对照组外,第8日起各组小鼠每日灌胃30 min后于低氧舱中模拟高海拔环境进行低氧暴露。第22日出舱称重后眼球采血处死小鼠,制备脾淋巴细胞悬液。MTT法检测各组小鼠脾淋巴细胞增殖能力、转化能力及NK细胞杀伤活力。ELISA检测各组小鼠血清白细胞介素(IL)-2含量。结果与对照组比较,模型组小鼠在处死前体质量明显下降,脾淋巴细胞增殖能力、转化能力、刺激指数及NK细胞杀伤活性均明显下降,血清IL-2含量明显降低(P<0.05,P<0.01);与模型组比较,各给药组小鼠脾淋巴细胞增殖能力、转化能力及NK细胞杀伤活性均明显升高(P<0.05,P<0.01),当归有效部位X组刺激指数明显升高,当归有效部位X、C组血清IL-2含量均明显升高(P<0.05,P<0.01)。结论当归不同有效部位对低氧暴露下的小鼠免疫功能具有一定的增强作用。
Objective To explore the intervention effect of active fraction of Angelica Sinensis Radix in mice under high altitude hypoxia condition. Methods Totally 72 healthy SPF mice were randomly divided into control group (K), model group (M), Rhodiola rosea group, and active fraction of Angelica Sinensis Radix groups (B, C, X). The mice were administerted corresponding treatment by gavage for 21 days. Control mice were given normal saline in same volume. From the 8th day, all mice excepted control mice were exposed to high altitude hypoxia cabin after 0.5 hour gavage treament. On the 22nd day, after got out of the cabin and their body weight were measured, mice were put to death through eyeball blood sampling to prepare splenic lymphocyte suspension. The proliferation and transformation capacities of lymphocyte cell and killing activity of NK cells were detected by MTT. The content of IL-2 in the serum of mice in each group were detected by ELISA. Results Compared with the control group, the body weight of mice, the proliferation and transformation capacities of lymphocyte cell, the killing activity of NK cells, and the content of IL-2 were all significantly decreased (P<0.05, P<0.01). Experiment tests showed that the proliferation and transformation abilities of lymphocyte cell and the killing activity of NK cells were all increased in the mice of group B, C, and X compared with those of the model group (P<0.05, P<0.01). The stimulate index of lymphocyte cell was raised after X intervention compared with those of the model group (P<0.05). The content of IL-2 in the serum was enhanced after intervention of active fraction C and X of Angelica Sinensis Radix compared with those of the model group (P<0.05, P<0.01). Conclusion Active fraction of Angelica Sinensis Radix shows increasing immunological function of mice exposed to hypoxia.