文献类型：期刊文献

中文题名：超滤-固体膜萃取-反萃取技术制备异甘草苷工艺研究

英文题名：Preparation of isoglycyrrhizin by ultrafiltration and solid membrane extraction and reverse extraction

作者：刘梅清[1];段瑞[3];孙晓燕[1];王继龙[1,2];冯晓莉[1,2];万玲娟[1];魏舒畅[1,2]

第一作者：刘梅清

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药制药工艺工程研究中心,甘肃兰州730000;[3]沈阳市食品药品检验所,辽宁沈阳110122

第一机构：甘肃中医药大学

年份：2022

卷号：53

期号：15

起止页码：4673

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金资助项目(82060721);甘肃省教育厅“双一流”科研重点项目(GSSYLXM-05)。

语种：中文

中文关键词：异甘草苷;超滤;固体膜;萃取;反萃取;甘草

外文关键词：isoglycyrrhizin;ultrafiltration;solid membrane;extraction;reverse extraction;Glycyrrhizae Radix et Rhizoma

摘要：目的 采用固体膜萃取技术制备甘草超滤液中的异甘草苷,以期得到该技术分离异甘草苷的工艺条件。方法 以异甘草苷的萃取和反萃取率为指标,考察膜萃取过程中三相体积流量及反萃取温度对异甘草苷萃取效果的影响。结果 最佳固体膜萃取条件为甘草超滤液体积流量94 mL/min、萃取剂体积流量188 mL/min、反萃取剂体积流量188 mL/min,反萃取温度35℃。在此条件下,异甘草苷的平均萃取率可达到97.15%,反萃取率达到80.41%,最终异甘草苷的转移率达到78.12%。结论 作为中药有效成分分离的一种新型技术,固体膜萃取技术具有高效、简单、萃取剂可循环利用等诸多优点,可为异甘草苷的分离制备提供一种新的适用技术。
Objective To prepare isoglycyrrhizin from Gancao(Glycyrrhizae Radix et Rhizoma, GRR) ultrafiltrate by solid membrane extraction technology, and obtain the technological conditions for the separation of isoglycyrrhizin. Methods The effects of three phase flow rate and reverse extraction temperature on the extraction efficiency of isoglycyrrhizin were investigated with the extraction and reverse extraction rates of isoglycyrrhizin as indexes. Results The optimal extraction conditions were as follows: flow rate of GRR ultrafiltrate was 94 mL/min, flow rate of extractant was 188 mL/min, flow rate of reverse extractant was 188 mL/min, and temperature of reverse extractant was 35 ℃. Under these conditions, the average extraction rate of isoglycyrrhizin was 97.15% and the reverse extraction rate was 80.41%, finally, the transfer rate of isoglycyrrhizin reached 78.12%. Conclusion As a new technology for the separation of the active components of traditional Chinese medicine, solid membrane extraction has many advantages, such as high efficiency, simplicity and recyclable extractant, which can provide a new suitable technology for the separation and preparation of isoglycyrrhizin.