文献类型：期刊文献

英文题名：Soy hull dietary fiber alleviates inflammation in BALB/C mice by modulating the gut microbiota and suppressing the TLR-4/NF-κB signaling pathway

作者：Yang, Lina[1,2];Lin, Qian[1];Han, Lin[1];Wang, Ziyi[1];Luo, Mingshuo[1];Kang, Wanrong[3];Liu, Jie[2];Wang, Jing[2];Ma, Tao[1];Liu, He[1]

第一作者：Yang, Lina

通信作者：Liu, H[1]

机构：[1]Bohai Univ, Coll Food Sci & Technol, Jinzhou 121013, Liaoning, Peoples R China;[2]Beijing Technol & Business Univ, Chin, Beijing 100048, Peoples R China;[3]Gansu Univ Chinese Med, Sci Res Ctr, Lanzhou 730000, Gansu, Peoples R China

第一机构：Bohai Univ, Coll Food Sci & Technol, Jinzhou 121013, Liaoning, Peoples R China

通信机构：[1]corresponding author), Bohai Univ, Coll Food Sci & Technol, Jinzhou 121013, Liaoning, Peoples R China.

年份：2020

卷号：11

期号：7

起止页码：5965

外文期刊名：FOOD & FUNCTION

收录：;EI(收录号：20204309381895);Scopus(收录号：2-s2.0-85088491439);WOS:【SCI-EXPANDED(收录号:WOS:000551326500022)】；

基金：This study was supported by the National Natural Science Foundation of China (Grant No. 31901680 and 31972031).

语种：英文

外文关键词：Glucose - Fourier transform infrared spectroscopy - RNA - Sulfur compounds - Diseases - Fibers - Mammals - Scanning electron microscopy

摘要：The present study is undertaken to assess the ability of insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) extracted from soy hulls to relieve colitis in dextran sulfate sodium (DSS) induced inflammatory bowel disease (IBD) in a BALB/C mouse model. We characterized dietary fiber (DF) structures by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Water retention capacity (WRC), water swelling capacity (WSC), oil adsorption capacity (OAC), glucose adsorption capacity (GAC), and the bile acid retardation index (BRI) were measured. The unique surface and chemical structural characteristics endowed DF with good absorption capacity and hydration ability, along with delayed glucose diffusion and absorption of bile acids. IBD was induced with a solution containing 5% DSS in male mice, which were administered a total oral dose of IDF (300 mg kg(-1)) and SDF (300 mg kg(-1)) three times per day after successful model establishment. All the mice were assessed weekly for weight change, diarrhea index, and fecal bleeding index. Levels of TLR-4 and NF-kappa B proteins were measured with western blotting analysis. Cytokine TNF-alpha in the serum was detected with an enzyme-linked immunosorbent assay (ELISA). Histological methods (H&E) were used to observe part of the mouse colon. The gut microbiota in the colonic contents was analyzed by 16S rRNA gene sequencing. DF decreased weight loss, diarrhea, and fecal bleeding, and also slowed serum TNF-alpha release. Increases in the levels of NF-kappa B proteins in inflamed colon tissue were also significantly suppressed by DF treatment. DF ameliorates the colitis induced decrease in gut microbiota species richness. The effect of SDF seemed clearer: the relative abundance ofBarnesiella,Lactobacillus,RuminococcusandFlavonifractorat the genus level was greater than that in the normal control group.