文献类型：期刊文献

中文题名：中药增骨丸对去卵巢大鼠骨髓IGF-1和IL-1β的影响

英文题名：Effect of Zenggu pill on IGF-1 and IL-1β in bone medullae of ovariectomized rats

作者：李宁[1];谢兴文[2];宋敏[1];张晓刚[1];宋鹏程[1]

第一作者：李宁

机构：[1]甘肃中医学院;[2]上海中医药大学

第一机构：甘肃中医药大学

年份：2004

卷号：17

期号：9

起止页码：524

中文期刊名：中国骨伤

外文期刊名：China Journal of Orthopaedics and Traumatology

收录：CSTPCD;;Scopus;PubMed

基金：甘肃省自然科学基金资助项目 (ZS0 1 1 A2 5 0 59 Y)

语种：中文

中文关键词：IL-1β;IGF-1;骨髓;增骨丸;大鼠;增高;正常;增加;影响;降低

外文关键词：Osteoporosis; Insulin-like growth factor I; Interleukin-1β; Chinese patent drugs

摘要：目的 :探讨去卵巢后大鼠骨髓上清中胰岛素样生长因子 1(IGF 1)和白细胞介素 1β(IL 1β)的变化及中药增骨丸对其影响。方法 :将 4 8只 8个月龄Wistar雌性大鼠随机分为正常对照组、模型组、乙烯雌酚组及 3种不同浓度的增骨丸组。每组 8只。正常组仅摘除一段肠系膜 ,其余 5组均做双侧卵巢切除术。术后 1周开始给药 ,连续用药 90d后取右侧股骨 ,剔净软组织 ,截取 1 5cm ,用 1mlPBS液将骨髓冲入DEPC处理过的试管中备用。分别测定骨髓中IGF 1和IL 1β的含量。 结果 :卵巢切除后大鼠骨髓上清中IGF 1的含量明显降低 ,而IL 1β的含量明显增高。经过雌激素及中药治疗能明显增高卵巢切除所致的IGF 1的降低 ,抑制IL 1β的增高 ,且其作用与剂量有关系。 结论 :增骨丸通过增加IGF 1的表达和降低IL 1β的表达而发挥防治骨质疏松症的作用。
Objective:To inquire into effects of Zenggu pill (ZGP) on bone medullae insulin-like growth factor-1 and Interleukin-1β in ovariectomized rats.Methods:Forty-eight healthy female Wistar rats were randomly divided into six groups:normal control group,model group,positive group and three ZGP groups (with high,moderate and low dosages respectively).The model group was excised a small part of mesentery and the other groups were ovariectomized bilaterally.They were put to death at the same time after three months.Then took right femur,measured the content of insulin-like growth factor-1 and Interleukin-1β in bone medullae by RIA and ELISA.Results:The content of insulin-like growth factor-1 in bone medullae of the high,moderate dosage of ZGP and positive control group rats were more higher than those in the model group (P<0.05),but the content of Interleukin-1β were much lower than that in the model group.Conclusion:The high and moderate ZGP can increase the content of insulin-like growth factor-1 and decrease Interleukin-1β in bone medullae.So they have the effect of prevention and treatment to postmenopausal osteoporosis,and the effects might be related to the level of doses of ZGP.