文献类型：期刊文献

中文题名：甘草总黄酮氨水提取及超滤纯化工艺研究

英文题名：Study on the Extraction of Total Flavonoids from Glycyrrhizae Radix et Rhizoma by Ammonia and the Purification of Ultrafiltration

作者：朱应怀[1,2];刘晓霞[1];宋晓春[1];魏舒畅[1,3];金辉[1];王继龙[1]

第一作者：朱应怀

机构：[1]甘肃中医药大学药学院;[2]甘肃中医药大学附属医院药学部;[3]甘肃省中医方药挖掘与创新转化重点实验室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：34

期号：4

起止页码：492

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目(81060345;81460608);甘肃省基础研究创新群体项目(1506RJIA034)

语种：中文

中文关键词：甘草;总黄酮;提取;超滤;转移率;除杂率

外文关键词：Glycyrrhizae Radix et Rhizoma; total flavonoids; extraction; ultrafiltration; transfer rate; removal rate

摘要：目的建立一种适合于工业化生产的甘草总黄酮提取和纯化方法。方法以甘草总黄酮的提取率为指标,采用正交试验确定氨水提取的最佳条件;并以甘草总黄酮的转移率和除杂率为指标,采用正交试验优化最佳超滤工艺参数。结果最佳提取条件:0.75%氨水24倍,提取3次,每次60 min,总黄酮平均提取率达83.5%;超滤结果表明:选用10 nm的无机陶瓷膜,在操作压力0.12 MPa和料液温度25℃条件下甘草总黄酮平均转移率和平均除杂率分别达到95.6%和23.3%。结论该联用技术简便可行,生产成本低,适合工业化生产。
OBJECTIVE To establish a suitable method for industrialized production of total flavonoids extraction and purification from Glycyrrhizae Radix et Rhizoma. METHODS The orthogonal test was used to determine the best extraction conditions within the extraction rate of total flavonoids as the index; and orthogonal experiment was used to optimize the parameters of ultrafiltration process taking the transfer rate of total flavonoids and the ratio of removal of impurity as indexes. RESULTS The best extraction conditions were as follows： 24 times of 0.75% ammonia, extract 3 times, 60 rain for each time, the average extraction rate of total flavonoids was 83.5%. Ultrafiltration results showed that： 10 nm of inorganic ceramic membrane, in the operating pressure of 0.12 MPa and liquid temperature of 25 ℃, the average transfer rate and average removal rate of total flavonoids were 95.6% and 23.3%. CONCLUSION The combined technique is simple and feasible, and the production cost is low, the method is suitable for industrial application.