文献类型：期刊文献

英文题名：Structural properties and hypoglycaemic activity of polysaccharides extracted from the fruits of Lycium barbarum L. using various extraction media

作者：Kou, Renbo[1,2,3];Zuo, Guanglei[2,3];Liu, Jianfei[2,3];Di, Duolong[1,2,3];Guo, Mei[1]

第一作者：Kou, Renbo

通信作者：Guo, M[1];Liu, JF[2];Liu, JF[3]

机构：[1]Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources & K, Lanzhou 730000, Peoples R China;[3]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources & K, Lanzhou 730000, Peoples R China;[3]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：188

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001026436100002)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21904130), the Science and Technology Program of Gansu Province (No. 20JR5RA559), and the Key Research and Development Program of the Ningxia Hui Autonomous Region (2021BEF02011).

语种：英文

外文关键词：Lycium barbarum polysaccharides; Extraction media; Hypoglycaemic activity; Structure analysis

摘要：The physical and chemical changes that occur during the preparation of polysaccharides are accompanied by changes in structure and biological activity. Different extraction media can affect the bioactivity of polysaccharides by altering their structural properties, including 1D composition, 2D conformation, and 3D morphology. In this study, polysaccharides from the fruits of Lycium barbarum L. were comparatively extracted using water (LBPs-W), 0.1 M HCl (LBPs-Ac), 0.1 M NaOH (LBPs-Al), 0.1% enzyme (LBPs-E), and 6% ionic liquid (LBPs-ILs) aqueous solution, respectively. The physicochemical properties and structures of the five LBPs were compared, and significant differences existed in their chemical components, monosaccharide composition, molecular weight distribution, glycosidic bond ratio, molecular conformation, and morphology. Of note, LBPs-ILs had the highest alpha-glucosidase, alpha-amylase, and aldose reductase (AR) inhibitory activities, which may be related to the different monosaccharide compositions, high branching degrees, rich porous structure, and triple helix structure. These results indicated that different media induce differences in structure and function in the process of polysaccharide extraction and dissolution. In addition, ILs are a promising and practical medium to extract hypoglycaemic LBPs from the fruits of Lycium barbarum L. and may be used to extract polysaccharides from other plant resources.