文献类型：期刊文献

英文题名：Anticolitis activity of chinese herbal formula Yupingfeng powder via regulating colonic enterochromaffin cells and serotonin

作者：Zang, Kai-hong[1];Rao, Zhi[2];Zhang, Guo-qiang[2];Qin, Hong-yan[2]

第一作者：臧凯宏

通信作者：Qin, HY[1]

机构：[1]Lanzhou Univ, Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Peoples R China.

年份：2015

卷号：47

期号：6

起止页码：632

外文期刊名：INDIAN JOURNAL OF PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-84948689422);WOS:【SCI-EXPANDED(收录号:WOS:000365695600010)】；

基金：This work was supported by the foundation of Key laboratory for gastrointestinal diseases of Gansu province in China (No. gswcky-2013-001).

语种：英文

外文关键词：Colonic inflammation; enterochromaffin cell; serotonin; ulcerative colitis

摘要：Objective: To investigate whether traditional Chinese herbal formula Yupingfeng (YPF) powder has an anti-inflammatory effect on colonic inflammation, and to explore the mechanism involved. Materials and Methods: YPF powder was orally administrated to trinitrobenzene sulfonic acid (TNBS)-induced colitis mice at the dose of 3, 6, and 12 g/kg/d for 7 consecutive days. Body weight, stool consistency, histopathological score, and myeloperoxidase (MPO) activity were tested to evaluate the effect of YPF powder on colonic inflammation while colonic enterochromaffin (EC) cell density and serotonin 5-hydroxytryptamine (5-HT) content were investigated to identify the effect of YPF powder on colonic 5-HT availability. Results: The results showed that the body weight of colitis mice was markedly decreased by 10, 12, 14, and 17% at 1, 3, 5, and 7 days (P < 0.05), whereas stool consistency score (3.6 vs. 0.4, P < 0.05), histopathological score (3.6 vs. 0.3, P < 0.05), and MPO activity (2.7 vs. 0.1, P < 0.05) in colitis mice were significantly increased compared to that of the normal mice; YPF powder treatment dose-dependently increased the body weight (7-13% increase) and decreased the stool consistency score (0.4-1.4 decrease), histopathological score (0.2-0.7 decrease), and MPO activity (0.1-0.9 decrease) in colitis mice. Colonic EC cell density (70% increase) and 5-HT content (40% increase) were markedly increased in colitis mice (P < 0.05), YPF powder treatment dose-dependently reduced EC cell density (20-50% decrease), and 5-HT content (5-27% decrease) in colitis mice. Conclusion: The findings demonstrate that the anti-inflammatory effect of YPF powder on TNBS -induced colitis may be mediated via reducing EC cell hyperplasia and 5-HT content. The important role of YPF powder in regulating colonic EC cell number and 5-HT content may provide an alternative therapy for colonic inflammation.