文献类型：期刊文献

中文题名：甘肃产黄管秦艽中龙胆苦苷的鉴别与含量测定

英文题名：Identification and Content Determination of Gentiopicroside in Gentiana officinalis H.Smith from Different Areas of Gansu

作者：余晓晖[1];赵磊[1];李阳[1];陈旅翼[1]

第一作者：余晓晖

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2010

卷号：27

期号：6

起止页码：504

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：甘肃省中医管理局2006年批准立项(06-GZK-29)

语种：中文

中文关键词：黄管秦艽;高效液相色谱法;龙胆苦苷;含量测定

外文关键词：Gentiana officinalis H.Smith; HPLC; gentiopicrin; content determination

摘要：目的对甘肃产黄管秦艽进行薄层鉴别,测定不同地区黄管秦艽的龙胆苦苷含量,以评价药材质量。方法采用薄层色谱法对黄管秦艽中的龙胆苦苷进行鉴别,并用高效液相色谱法对其进行含量测定。结果龙胆苦苷在23.5～70.5μg内线性关系良好(r=0.9993),平均加样回收率为98.59%(RSD=1.12%,n=6)。结论薄层色谱法斑点清晰,专属性强;HPLC简便、准确、重复性好。甘肃不同地区黄管秦艽龙胆苦苷的含量有差异,但均高于中国药典2005版规定。本试验可为人工栽培秦艽、保护秦艽野生资源提供科学依据。
OBJECTIVE To evaluate the quality of Gentiana officinalis H.Smith from different areas in Gansu province by indentification and content determination of gentiopicrin.METHODS The gentiopicrin in Gentiana officinalis H.Smith was identified by TLC and determined by HPLC.RESULTS The calibration curve of gentiopicrin was linear within 23.5～70.5 μg（r=0.999 3）,and the average recovery rate was 98.59%（RSD=1.12%,n=6）.CONCLUSION The TLC identification method has been proved specific,and HPLC method has been proved simple,accurate and reproducible.Moreover,the results showed that the contents of gentiopicrin in Gentiana officinalis H.Smith from different areas of Gansu are different,but higher than the standard required by Chinese Pharmacopoeia.This study provides a scientific basis for artificial cultivation of Gentiana officinalis and protection of wild Gentiana officinalis.