文献类型：期刊文献

中文题名：甘肃黄芩、苦参、金银花对深部酵母样真菌感染的体外抑菌试验研究

英文题名：Bacteriostasis Test in vitro of Deep Yeast-like Fungi by Radix Scutellariae,Radix Sophorae Flavescentis and Flos Lonicerae in Gansu

作者：张美玲[1];魏莲花[2];田洹[1];王宏伟[1];黄亚乐[1]

第一作者：张美玲

机构：[1]甘肃中医学院附属医院检验科,甘肃兰州730020;[2]甘肃省临床检验中心,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2009

卷号：19

期号：2

起止页码：149

中文期刊名：中华医院感染学杂志

外文期刊名：Chinese Journal of Nosocomiology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：甘肃省科技攻关计划项目(2GS064-A43-020-28)

语种：中文

中文关键词：中草药;抑菌物质;深部感染;酵母样真菌

外文关键词：Traditional chinese materia medica;Bacteriostatic substance;Deep infection; Yeast-like fungus

摘要：目的探讨甘肃黄芩、苦参、金银花中抑制深部酵母样真菌感染的有效活性成分。方法将甘肃黄芩、苦参、金银花洗净、烘干、粉碎成（80目）细粉，收集临床分离酵母样真菌5种共8株，接种于90mm萨布罗平板，在无菌条件下，将平板打孔为6mm，每孔填入黄芩、苦参、金银花药粉25．73mg，在药粉中滴加无菌蒸馏水3～5滴，35℃培养24～48h，观察抑菌环大小。结果黄芩药粉对5种酵母样真菌抑菌环直径分别为16．0、13．5、13．0、13．0、10．5mm；苦参粉和金银花粉抑菌环为0。结论甘肃黄芩中含有抑制酵母样真菌的有效活性成分，甘肃苦参、金银花中无有效活性成分。
OBJECTIVE To find the active principle in Radix Scutellariae, Radix Sophorae Flavescentis and Flos Lonicerae in Gansu inhibiting yeastqike fungi of deep infection. METHODS Radix Scutellariae, Radix Sophorae Flavescentis and Flos Lonieerae were separately cleaned, toasted and grinded to fine power. Five kinds of （8 strains） yeast-like fungi that were collected and spread into 90mm Sabouraud plate. On aseptic condition, bored holes on Sabouraud plate with the drilling instrument of 6ram diameter, and filled every holes with 25.73mg fine powder of Radix Scutellariae,Radix Sophorae Flavescentis and Flos Lonicerae, and added 3 to 5 drops of distilled water in the medicinal powder. Under 35℃, the bacteria were cultured for 24 to 48 hours, and the size of bacteriostatic ring was observed. RESULTS The diameters of the bacteriostatic ring of Radix Scutellariae powder inhibiting five kinds of yeast-like fungi were separately 16. 0mm, 13. 5mm, 13. 0mm, 13. 0mm and 10. 5mm, respectively, and the diameter of Radix Sophorae Flavescentis and Flos Lonicerae powder was 0. CONCLUSIONS There is the active principle inhibiting yeast-like fungi in Radix Scutellariae, but not that in Radix Sophorae Flavescentis and Flos Lonicerae.