文献类型：期刊文献

英文题名：Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway

作者：Xu, Chao[1];Ni, Su[2];Zhuang, Chao[3];Li, Chenkai[2];Zhao, Gongyin[3];Jiang, Shijie[3];Wang, Liangliang[3];Zhu, Ruixia[3];van Wijnen, Andre J.[4];Wang, Yuji[3,4,5]

第一作者：Xu, Chao

通信作者：Wang, YJ[1];van Wijnen, AJ[2];Wang, YJ[2];Wang, YJ[3]

机构：[1]Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Trauma Ctr, 29 Xinglong Alley, Changzhou 213003, Jiangsu, Peoples R China;[2]Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Med Res Ctr, 29 Xinglong Alley, Changzhou 213003, Jiangsu, Peoples R China;[3]Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Dept Orthoped, 29 Xinglong Alley, Changzhou 213003, Jiangsu, Peoples R China;[4]Mayo Clin, Dept Orthoped Surg & Biochem & Mol Biol, Rochester, MN 55905 USA;[5]Gansu Univ Chinese Med, Affiliated Hosp 3, Dept Orthoped, 222 Silong Rd, Baiyin 730900, Peoples R China

第一机构：Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Trauma Ctr, 29 Xinglong Alley, Changzhou 213003, Jiangsu, Peoples R China

通信机构：[1]corresponding author), Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Dept Orthoped, 29 Xinglong Alley, Changzhou 213003, Jiangsu, Peoples R China;[2]corresponding author), Mayo Clin, Dept Orthoped Surg & Biochem & Mol Biol, Rochester, MN 55905 USA;[3]corresponding author), Gansu Univ Chinese Med, Affiliated Hosp 3, Dept Orthoped, 222 Silong Rd, Baiyin 730900, Peoples R China.|[10735]甘肃中医药大学;

年份：2021

卷号：23

期号：1

外文期刊名：ARTHRITIS RESEARCH & THERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000616422400003)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (81171680 to Y.W.), the project of Jiangsu province (BE2015632 to Y.W.), the Natural Science Foundation of the Jiangsu for Youth (BK20180182 to S.N.), the Changzhou Science and Technology Program (CJ20180057 to S.N; QN201930 to C.L.), and the China Postdoctoral Science Foundation (2019M651898 to S.N.).

语种：英文

外文关键词：Angelica sinensis polysaccharide; Autophagy; Chondrocytes; Osteoarthritis

摘要：ObjectiveChondrocyte apoptosis plays a vital role in osteoarthritis (OA) progression. Angelica sinensis polysaccharide (ASP), a traditional Chinese medicine, possesses anti-inflammatory and anti-apoptotic properties in chondrocytes. This study aimed to determine the protective role of ASP on sodium nitroprusside (SNP)-induced chondrocyte apoptosis, and explore the underlying mechanism.MethodHuman primary chondrocytes isolated from the articular cartilage of OA patients were treated with SNP alone or in combination with different doses of ASP. Cell viability and apoptosis were assessed, and apoptosis-related proteins including Bcl-2 and Bax were detected. Autophagy levels were evaluated by light chain 3 (LC3) II immunofluorescence staining, mRFP-GFP-LC3 fluorescence localization, and western blot (LC3II, p62, Beclin-1, Atg5). Meanwhile, activation of the ERK 1/2 pathway was determined by western blot. The autophagy inhibitors, 3-methyladenine (3-MA), chloroquine (CQ), and a specific inhibitor of ERK1/2, SCH772984, were used to confirm the autophagic effect of ASP.ResultsThe results showed that SNP-induced chondrocyte apoptosis was significantly rescued by ASP, whereas ASP alone promoted chondrocyte proliferation. The anti-apoptotic effect of ASP was related to the enhanced autophagy and depended on the activation of the ERK1/2 pathway.ConclusionASP markedly rescued SNP-induced apoptosis by activating ERK1/2-dependent autophagy in chondrocytes, and it made ASP as a potential therapeutic supplementation for OA treatment.