文献类型：期刊文献

中文题名：甘肃不同产地当归ITS序列分析

英文题名：ITS Sequence Analysis of Angelica from Different Source in Gansu Province

作者：楚惠媛[1];金建文[1];李海龙[1];李沛清[1];陈彻[1];安方玉[1]

第一作者：楚惠媛

机构：[1]甘肃中医学院基础部,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2009

卷号：16

期号：8

起止页码：43

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃省教育厅科研项目(014-02)

语种：中文

中文关键词：当归;ITS;序列分析;道地性;甘肃

外文关键词：Angelica; intemal transcribed spacer; sequence analysis geoherbalism; Gansu

摘要：目的建立甘肃产当归功效的分子系统学基础,为甘肃产当归"道地性"提供分子依据。方法采用PCR技术获得ITS基因,进行测序,经软件进行统计分析,计算各样本间的遗传距离。结果甘肃岷、漳两县当归ITS序列差异极小,在0.0000～0.0083之间,岷县5个样品与漳县DG9号样品在586位都为C,589位都为T,而漳县其他3个样品在这2个位点分别为T和G。结论ITS序列特征可以作为当归种间鉴别的有效分子标记,当归ITS序列第586位和589位2个位点碱基可能成为"岷当归"的碱基鉴别位点。
Objective To establish the basis of molecular systematics of Angelica from different areas of Gansu province so as to offer molecular basis of identification Gansu-produced Angelica with geoherbalism. Methods The ITS gene of Angelica was obtained by PCR and sequenced to be analyzed by DNAStar software to calculate the genetic distance among different specimens. Results The ITS sequence of Angelica between Min County and Zhang County of Gansu Province shows trivial differences with the range of 0.000 0-0.008 3. All 5 Min County-produced Angelica specimens have same bases at 586 site with ＂C＂ and 589 site with ＂T＂ with Zhang County-produced Angelica DG9 specimen, whereas the 586 site and 589 site bases of the other 3 Zhang County-produced Angelica specimens are ＂T＂ and ＂G＂ respectively. Conclusion The ITS sequence characteristics can be recognized as effective molecular marker of Angelica, and both 586 site base and 589 site base of the ITS sequence of Angelica can be recognized as identification bases of Min County-produced Angelica.