文献类型：期刊文献

英文题名：Icariin suppresses nephrotic syndrome by inhibiting pyroptosis and epithelial-to-mesenchymal transition

作者：Duan, Shuwen[1];Ding, Zhaoran[1];Liu, Can[1];Wang, Xiaohui[1];Dai, Enlai[1]

第一作者：Duan, Shuwen

通信作者：Dai, EL[1]

机构：[1]Gansu Univ Tradit Chinese Med, Dept Tradit Chinese & Western Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Dept Tradit Chinese & Western Med, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：19

期号：7

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-85198609138);WOS:【SCI-EXPANDED(收录号:WOS:001267555600044)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (82160852), and Star of Innovation of Excellent Postgraduates in Gansu Province (2022CXZX-737). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Context Nephrotic syndrome(NS) has emerged as a worldwide public health problem. Renal fibrosis is the most common pathological change from NS to end-stage renal failure, seriously affecting the prognosis of renal disease. Although tremendous efforts have been made to treat NS, specific drug therapies to delay the progression of NS toward end-stage renal failure are limited. Epimedium is generally used to treat kidney disease in traditional Chinese medicine. Icariin is a principal active component of Epimedium.Methods We used Sprague Dawley rats to establish NS models by injecting doxorubicin through the tail vein. Then icariin and prednisone were intragastric administration. Renal function was examined by an automatic biochemical analyzer. Pathology of the kidney was detected by Hematoxylin-Eosin and Masson staining respectively. Furthermore, RT-PCR, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Western Blot and Terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling staining were employed to detect the proteins related to pyroptosis and EMT. HK-2 cells exposed to doxorubicin were treated with icariin, and cell viability was assessed using the MTT. EMT was assessed using Enzyme-Linked Immunosorbent Assay and Western Blot.Results The study showed that icariin significantly improved renal function and renal fibrosis in rats. In addition, icariin effectively decreased NOD-like receptor thermal protein domain associated protein 3,Caspase-1, Gasdermin D, Ly6C, and interleukin (IL)-1 beta. Notably, treatment with icariin also inhibited the levels of TGF-beta, alpha-SMA and E-cadherin.Discussion and conclusions It is confirmed that icariin can improve renal function and alleviate renal fibrosis by inhibiting pyroptosis and the mechanism may be related to epithelial-to-mesenchymal transition. Icariin treatment might be recommended as a new approach for NS.