文献类型：期刊文献

英文题名：Evaluation of a modified rat model for functional dyspepsia

作者：Liang, Qiankun[1];Yan, Yuan[1];Mao, Lanfang[2];Du, Xiaojuan[2];Liang, Jingjing[1];Liu, Junhong[2];Wang, Longde[2];Li, Hongfang[1,3]

第一作者：Liang, Qiankun

通信作者：Li, HF[1]

机构：[1]Lanzhou Univ, Coll Basic Med, Dept Physiol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Dept Gastroenterol, Affiliated Hosp, Lanzhou, Gansu, Peoples R China;[3]Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Coll Basic Med, Dept Physiol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Coll Basic Med, Dept Physiol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2018

卷号：24

期号：4

起止页码：228

外文期刊名：SAUDI JOURNAL OF GASTROENTEROLOGY

收录：;Scopus(收录号：2-s2.0-85051233581);WOS:【SCI-EXPANDED(收录号:WOS:000440627500005)】；

基金：Supported by the Natural Science Foundation of China, No. 81360540.

语种：英文

外文关键词：Brain-gut axis; brain-gut peptides; functional dyspepsia; gastrointestinal motility

摘要：Background/Aim: The purpose of this study was to establish a modified rat model with functional dyspepsia (FD) and analyze the changes in gastrointestinal motility and brain-gut peptide levels in serum and brain-gut axis. Materials and Methods: Male Wistar rats were divided into control group (Con) and FD model group. FD model was established by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue until gastrointestinal motility disorder appeared, and then levels of motilin, leptin, cholecystokinin (CCK), and vasoactive intestinal peptide (VIP) were detected in serum by enzyme linked immunosorbent assay and in duodenum, antrum, and hypothalamus by immunohistochemistry, reverse transcriptase-polymerase chain reaction, and Western blot. Results: The results showed rates of intestinal propulsion and gastric emptying slowed down markedly compared to Con (P < 0.05), the gastrointestinal electric activity attenuated, and migrating motor complex (MMC) interrupted in the model group. The levels of leptin and VIP markedly increased, but motilin decreased as compared to the Con (P < 0.05) in serum and in the above tissues. It is interesting that the level of CCK decreased in the antrum and duodenum but increased in the hypothalamus as compared to Con (P < 0.05). Conclusions: The modified rat model meets the diagnostic criteria of FD and can be used as a method for studying FD in animals.