文献类型：期刊文献

中文题名：藤黄健骨胶囊对膝骨关节炎大鼠血细胞变化及炎症因子表达的影响

英文题名：Effect of Tenghuangjiangu capsule on the expression of blood cell and inflammatory cytokines in rats with knee osteoarthritis

作者：颜春鲁[1];安方玉[2];蒋国凤[1];赵崇博[3];李嘉进[4];李孟翰[4];郭丹[3]

第一作者：颜春鲁

机构：[1]甘肃中医药大学中西医结合学院,兰州730000;[2]甘肃中医药大学教学实验实训中心,兰州730000;[3]甘肃中医药大学临床医学院,兰州730000;[4]甘肃中医药大学中医临床学院,兰州730000

第一机构：甘肃中医药大学中西医结合学院

年份：2020

卷号：37

期号：6

起止页码：84

中文期刊名：生物学杂志

外文期刊名：Journal of Biology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：甘肃省高等学校创新能力提升项目(2019A-080);甘肃省高校重大疾病分子医学与中医药防治研究重点实验室开放基金项目(FZYX17-18-10)。

语种：中文

中文关键词：藤黄健骨胶囊;膝骨关节炎;大鼠;血细胞;炎症因子

外文关键词：Tenghuangjiangu capsule;knee osteoarthritis;rat;blood cell;inflammatory cytokines

摘要：观察藤黄健骨胶囊对膝骨关节炎(knee osteoarthritis,KOA)大鼠右后足足跖厚度、血细胞变化及炎症因子IL-6和IL-2表达的影响,并探讨藤黄健骨胶囊治疗KOA的作用机制。将60只SPF级SD雌性大鼠随机分为假手术组、KOA模型组、阳性对照组和藤黄健骨胶囊高、中、低剂量组。改良Hulth法复制KOA大鼠模型。假手术组和KOA模型组给予等体积蒸馏水灌胃,阳性对照组给予仙灵骨葆胶囊(0.27 g/kg),藤黄健骨胶囊高、中、低剂量组给予藤黄健骨胶囊灌胃(0.36、0.18和0.09 g/kg)。末次给药结束24 h后,HE染色法观察各组大鼠软骨组织的病理形态学变化,并进行Mankin评分。采用游标卡尺测量法测定各组大鼠右后足足跖的厚度、全血自动分析仪检测各组大鼠白细胞(White blood cell,WBC)、中性粒细胞(Neutrophils,N)、淋巴细胞(Lymphocyte,L)、单核细胞(Monocytes,M)和嗜酸粒细胞(Eosinophils,EOS)的变化,ELISA法测定各组大鼠炎症因子IL-6和IL-2的含量。结果表明藤黄健骨胶囊各剂量组能显著降低KOA模型大鼠右后足足跖厚度、WBC、N/L和白细胞介素-6(Interleukin,IL-6)等指标,藤黄健骨胶囊中、高剂量组能够显著降低KOA模型大鼠Mankin评分和N数目,显著升高KOA模型大鼠的L数目,藤黄健骨胶囊高剂量组能够显著降低KOA模型大鼠白细胞介素-2(Interleukin,IL-2)含量(P<0.05)。这说明藤黄健骨胶囊可以减轻KOA疼痛和延缓软骨退变,可能与减少炎症细胞数目和炎症细胞因子含量有关。
To observe the plantar thickness of right posterior foot,the number of blood cells,the levels of IL-6 and IL-2 in rats with knee osteoarthritis(KOA),the mechanism of Tenghuangjiangu capsule was explored.Sixty SD female rats were randomly divided into a shame control group,KOA medel group,a positive control group,Tenghuangjiangu capsule high-dose,middle-dose and low-dose grous.The modified Hulth method was used to make KOA models for 6 weeks.The rats in shame control group and model group were treated with normal saline,while the rats in Tenghuangjiangu capsule(high-dose,middle-dose,low-dose)groups received Tenghuangjiangu capsule(0.36,0.18 and 0.09 g/kg),respectively,and which in the positive control group was treated with Xianlinggubao capsule(0.27 g/kg).After intervening,rats in each group were killed by femoral artery blood,and keen cartilage of rats were obtained,the pathological change was observed by HE staining method,and Mankin score was evaluated.The vernier caliper was adopted to observe the plantar thickness of right posterior foot,the whole blood automatic analyzer was applied to measure the number of blood cells,and the ELISA method was used to check the levels of IL-6 and IL-2.Results showed that the rats in Tenghuangjiangu capsule treatment group had the significant difference in decreasing the numbers of WBC,N/L,the level of IL-6 and the thickness of right rear fully plantar,while the rats in the middle group and in high group showed significant difference in dropping the Mankin score,the N number and increasing the L numbers;the level of IL-2 was decreased in high-group rats(P<0.05).The results indicated that Tenghuangjiangu capsule could relieve KOA pain and delay cartilage degeneration,which might be related to the decrease of the numbers of inflammatory cells and the expression of inflammatory cytokines.