文献类型：期刊文献

中文题名：当归酒炙增强活血化瘀作用的血清代谢组学研究

英文题名：Serum metabolomics of wine-processed Angelicae Sinensis Radix in enhancing blood-activating and stasis-resolving effects

作者：付晓艳[1];高广淼[1];杨秀娟[1,2];邓毅[1,2];张金保[1];巩子汉[3]

第一作者：付晓艳

机构：[1]甘肃中医药大学;[2]甘肃中医药大学中药药理与毒理学重点实验室,兰州730000;[3]宁夏回族自治区中医医院暨中医研究院,银川750021

第一机构：甘肃中医药大学

年份：2026

卷号：38

期号：1

起止页码：13

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：;北大核心:【北大核心2023】；

基金：西北中藏药省部共建协同创新中心开放基金(Xbzzy-2022-08);甘肃省高等学校科研项目(2021B-157);甘肃省中药炮制技术传承基地建设揭榜挂帅项目(2022-13);宁夏自然科学基金(2024AAC03727)。

语种：中文

中文关键词：当归;酒当归;急性血瘀证;代谢组学;作用机制

外文关键词：Angelicae Sinensis Radix;wine-processed Angelicae Sinensis Radix;acute blood stasis syndrome;metabolomics;mechanism of action

摘要：探讨当归酒炙对急性血瘀模型大鼠的活血化瘀作用及其作用机制。连续灌胃给予大鼠当归、酒当归水煎液7 d后,通过冰水浴加皮下注射盐酸肾上腺素(0.8 mg/kg)的复合因素法制备急性血瘀大鼠模型,通过测定血液流变学指标及凝血酶原时间、活化部分凝血活酶时间、凝血酶时间和纤维蛋白原含量评价当归酒炙增强活血化瘀的效果;采用LC-MS/MS非靶向代谢组学技术结合多元统计比较分析各组大鼠血清内源性代谢物,筛选差异代谢物并构建其代谢通路。结果显示,当归、酒当归均可明显改善急性血瘀模型大鼠血液流变学特征,有效延长凝血酶原时间、活化部分凝血活酶时间、凝血酶时间,显著降低纤维蛋白原含量。代谢组学分析表明,有113个差异代谢物与血瘀证的密切相关,其中18个差异代谢物在当归干预后显著回调,主要参与亚油酸代谢和不饱和脂肪酸的生物合成;22个差异代谢物在酒当归干预后显著回调,调节视黄醇代谢、亚油酸代谢、花生四烯酸代谢和苯丙氨酸、酪氨酸和色氨酸生物合成等4条代谢途径。综上所述,当归酒炙后对急性血瘀模型大鼠的活血化瘀效果优于生当归,其作用机制可能与调节视黄醇代谢、花生四烯酸代谢及色氨酸代谢有关。
This study aims to explore the blood-activating and stasis-resolving effects of wine-processed Angelicae Sinensis Radix(WP-ASR)on rats with acute blood stasis model and reveal underlying mechanism.After continuous intragastric administration with aqueous decoctions of Angelicae Sinensis Radix(ASR)and WP-ASR for seven days,an acute blood stasis model was established using a compound method involving ice-water bath immersion combined with subcutaneous injection of epinephrine hydrochloride(0.8 mg/kg).The blood-activating and stasis-resolving efficacy of WP-ASR was evaluated by measuring hemorheological parameters,prothrombin time,activated partial thromboplastin time,thrombin time,and fibrinogen levels.Furthermore,LC-MS/MS non-targeted metabolomics technology coupled with multivariate statistical analysis was employed to compare endogenous metabolites in rat serum across groups.Differential metabolites were screened,and their associated metabolic pathways were constructed.The results showed that ASR and WP-ASR could significantly improve the hemorheological characteristics of the acute blood stasis rat model,prolong prothrombin time,activated partial thromboplastin time,thrombin time and reduce fibrinogen content.Metabolomics analysis showed that 113 differential metabolites were closely related to the blood stasis syndrome,among which 18 differential metabolites were significantly attenuated after ASR intervention,mainly involving linoleic acid metabolism and unsaturated fatty acid biosynthesis.Twenty-two differential metabolites were significantly attenuated after WP-ASR intervention,regulating retinol metabolism,linoleic acid metabolism,arachidonic acid metabolism,and phenylalanine,tyrosine,and tryptophan biosynthesis.In summary,the effects of WP-ASR on acute blood stasis model rat are better than those of ASR,and its mechanism may be related to regulating retinol metabolism,arachidonic acid metabolism and tryptophan metabolism.