文献类型：期刊文献

中文题名：当归多糖对糖尿病大鼠脂代谢及脂肪因子的影响

英文题名：Influence of Angelica Polysaccharide on Lipid Metabolism and Adipokine in Diabetic Rats

作者：孟祥云[1,2];张之弘[3];汪永锋[4];郭树明[1];王晓怀[1,2];杨丽霞[2]

第一作者：孟祥云

机构：[1]甘肃省中医院,甘肃兰州730050;[2]甘肃省中医药研究院;[3]张掖市甘州区人民医院;[4]甘肃中医药大学

第一机构：甘肃省中医院,甘肃兰州730050

年份：2021

卷号：34

期号：11

起止页码：33

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金(81560764);甘肃省中医药管理局科研项目(GZK-2016-48、GZK-2017-4);甘肃省中医院院级课题(院2017-10);兰州市科技发展指导性项目(2020-ZD-34)。

语种：中文

中文关键词：糖尿病,2型;血脂;脂肪细胞因子;当归多糖;大鼠

外文关键词：type 2 diabetes mellitus;blood lipid;adipokine;AP;rats

摘要：目的:探讨当归多糖对糖尿病大鼠脂代谢及脂肪因子的影响。方法:将经链脲佐菌素(strep-tozotocin,STZ)诱导的2型糖尿病大鼠随机分为模型组、正常组、吡格列酮组及当归多糖低、中、高剂量组,每组10只。模型组、正常组给予生理盐水10 mL/kg灌胃,当归多糖各剂量组分别予400、200、100 mg/kg药物灌胃,吡格列酮组给予吡格列酮10 mg/kg灌胃。每日灌胃1次,灌胃4周后观察干预期间及干预后大鼠的一般情况及体质量变化情况,检测大鼠血脂[总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)]、脂肪细胞因子[瘦素(leptin,LEP)、脂联素(adiponectin,ADPN)、抵抗素(RSTN)]以及脂肪细胞变化情况。结果:模型组大鼠毛色枯槁,活动量下降,二便增多。经当归多糖干预后各组大鼠与吡格列酮组活动量基本正常、毛色光泽,大小便较模型组好转,较正常组偶有增多。与正常组比较,模型组大鼠体质量增长速度较快;与模型组比较,各药物干预组大鼠体质量增长速度均较慢。药物干预各组均能降低血清TC、TG、LDL,LEP和RSTN含量,提高HDL及ADPN水平;其中当归多糖高剂量组、吡格列酮组与模型组比较差异显著(P<0.01)。当归多糖各剂量组随着给药剂量的增加,脂肪细胞大小均匀,排列逐渐致密整齐,细胞截面基本恢复正常,空泡及囊腔样病变明显减轻。结论:当归多糖能调节2型糖尿病大鼠脂代谢水平,增加血清脂肪因子ADPN含量,降低LEP、RSTN含量,且对脂肪组织有一定的修复和保护作用。
Objective:To explore the effects of angelica polysaccharide(AP)on lipid metabolism and adipokine in diabetic rats.Methods:All STZ-induced DM rats were randomized into the model group,the normal group,pioglitazone group,low,moderate and high dosages groups of AP,ten rats in each group.The model group and the normal group were drenched with physiological saline,10mL/kg,different dosages groups of AP accepted intragastric administration of 400,200 and 100mg/kg AP,and pioglitazone group was given with pioglitazone,10mg/kg,once each day.After four weeks of intragastric administration,to observe general conditions and the changes of body mass during the intervention period and after the intervention,to detect blood lipid(TC,TG,LDL,HDL),adipocytokines(LEP,ADPN,RSTN)and the changes of fat cells of the rats.Results:Rat hair withered,the activity decreased,stool and urine increased in the model group.After intervention,compared with pioglitazone group,the activity was basically normal,the hair color was shiny in different dosages groups of AP,stool and urine were better than these in the model group,and increased occasionally compared with these of the blank group.Compared with the blank group,the body mass of rats increased rapidly in the model group;compared with the model group,the growth rate of body mass of the rats was slower in drug intervention groups.Different groups of drug intervention could lower the contents of TC,TG,LDL,LEP and RSTN,raise the levels of HDL and ADPN;among them,the difference was significant when high dosage group of AP and pioglitazone group were compared with the model group(P<0.01).As the medication dose increased in different dosages groups of AP,the size of adipocytes was uniform,the arrangement was gradually dense and neat,the cell cross section basically returned to normal,and the vacuole and cystic lesions were significantly reduced.Conclusion:AP could regulate the level of lipid metabolism in type 2 diabetic rats,increase the content of serum adipokine ADPN,reduce the content of LEP and RSTN,and have certain repair and protection effect on adipose tissue.