文献类型：期刊文献

中文题名：苓甲抗癌复方提取物在荷瘤小鼠中抗肿瘤免疫应答机制

英文题名：The anti-tumor immune response mechanism of Lingjia anti-tumor medicine in tumor-bearing mice

作者：潘燕龙[1,2];张文超[1];陈婧[1];周程[1];马银杏[3];李成义[4];王明伟[4];王勤[1]

第一作者：潘燕龙

机构：[1]兰州大学生命科学学院,甘肃兰州730000;[2]甘肃中医药大学科研实验中心,甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730000;[3]法国国家科学研究院UMR8126,巴黎第十一大学,古斯塔夫路西研究所,法国巴黎;[4]甘肃中医药大学药学院,甘肃兰州730000

第一机构：兰州大学生命科学学院,甘肃兰州730000

年份：2016

卷号：24

期号：11

起止页码：1699

中文期刊名：现代肿瘤医学

外文期刊名：Journal of Modern Oncology

收录：CSTPCD

基金：甘肃省科技支撑项目(编号:1104FKCA123)

语种：中文

中文关键词：肿瘤免疫;中药复方;调节性T细胞;FOXP3

外文关键词：tumor immunity;traditional chinese medicine;regulatory T cells;FOXP3

摘要：目的:初步探究苓甲抗癌复方提取物(LAM)在荷瘤小鼠中抗肿瘤免疫应答机制。方法:LAM治疗荷瘤小鼠,检测小鼠生长、瘤块体积与重量的变化;流式细胞术和ELISA分析小鼠脾脏中Treg细胞、NK细胞和各种细胞因子;Western blot分析LAM对Treg细胞相关蛋白作用。结果:LAM抑制荷瘤小鼠肿瘤的生长,引发肿瘤坏死,抑制肿瘤促增殖蛋白Ki67的表达,降低血液中CA153的含量;降低脾脏中Treg细胞比例和血清中TGF-β与IL-10的含量,提高IL-2、IFN-γ、穿孔素、颗粒酶B的含量和NK细胞的比例。结论:LAM抑制肿瘤的生长,并抑制Treg细胞的数量与功能,提高机体的免疫功能。
Objective: To investigate the mechanism of anti- tumor immune responses of tumor- bearing mice by Ling Jia compound extract( LAM). Methods: Examine the effect of LAM on mouse growth and tumor masses size,weight changes. Examine the effect of LAM on Treg cells,NK cells and various cytokines by flow cytometry and ELISA. Examine the effect of LAM on the key proteins of Treg cells by western blot. Results: LAM inhibited tumor growth and Ki67 expression,induced tumor necrosis,reduced CA153 in serum. The most important finding is that LAM can reduce the rate of Treg cells in spleen lymphocytes and the content of TGF- β and IL- 10 in serum and culture,leading to increase the content of NK cells,IL- 2,IFN- γ,Perforin,Granzyme B. Conclusion: LAM could inhibit tumor growth and improve immune function by inhibition of Treg cells numbers and function.