文献类型：期刊文献

英文题名：Changpu Yujin Tang mitigates tourette syndrome by enhancing mitophagy and suppressing NLRP3 inflammasome-mediated pyroptosis

作者：Huang, Shuang[1];Huang, Liwei[1];Li, Mengxue[1];Sun, Mingyang[1];Sun, Kexin[1];Wei, Xing[1];Jiang, Bing[2];He, Yuezhen[3];Xue, Fuchun[1];Gao, Lv[4];Lu, Manqi[5];Shang, Jing[1];Shi, Zhenggang[1]

第一作者：Huang, Shuang

通信作者：Shi, ZG[1]

机构：[1]Gansu Univ Chinese Med, Clin Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Dept Integrated Chinese & Western Med, Lanzhou 730000, Peoples R China;[3]First Hosp Lanzhou Univ, Dept Tradit Chinese Med, Lanzhou 730000, Peoples R China;[4]Shanxi Univ Chinese Med, Clin Med Coll 3, Pediat Teaching & Res Dept, Taiyuan 140100, Peoples R China;[5]Shanghai Univ Tradit Chinese Med, LongHua Hosp, Shanghai 200000, Peoples R China

第一机构：甘肃中医药大学中医临床学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735ccd4a8840d96ab71]甘肃中医药大学中医临床学院;[10735]甘肃中医药大学;

年份：2025

卷号：230

期号：6

外文期刊名：IMMUNOBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001586144000001)】；

基金：This work was supported by the National Natural Science Foundation of China (NO. 82460951) and the Research and Development Fund Project of Gansu University of Chinese Medicine (NO. 20YF3FA041) .

语种：英文

外文关键词：Tourette syndrome; Changpu Yujin Tang; PINK1/Parkin-mediated mitophagy; NLRP3 inflammasome; Pyroptosis

摘要：Background: Changpu Yujin Tang (CPYJT) is an effective Chinese herbal compound for treating Tourette syndrome (TS). However, its precise molecular mechanisms remain to be fully elucidated. Methods: 105 SD rats were randomly divided into the Control (n = 15) and the TS (n = 90) groups. The TS group was induced by intraperitoneal injection of 3,3 '-iminodipropionitrile. After successful modeling, the TS group was further divided into 6 subgroups (n = 15 in each group): the Model group, the Tiapride group, the CPYJT group, the Rapamycin (RAPA) group, the 3-methyladenine (3-MA) group, and the CPYJT +3-MA group, and were treated with the corresponding drugs for 4 weeks. Results: Compared with the Control group, rats in the Model group showed increased stereotyped and motor behaviors, damage to striatal neuronal cells and mitochondrial ultrastructure, decreased PINK1/Parkin-mediated mitophagy, and activation of NLRP3 inflammasome. CPYJT reduced stereotyped and motor behaviors, attenuated neuronal cell damage, and repaired mitochondrial pathology in the TS rats. Furthermore, both CPYJT and RAPA enhanced PINK1/Parkin-mediated mitophagy, eliminated ROS accumulation, and inhibited NLRP3 inflammasome activation. Notably, CPYJT counteracted 3-MA's inhibitory effect on PINK1/Parkin-mediated mitophagy, thereby suppressing NLRP3 inflammasome activation and pyroptosis. Conclusion: CPYJT inhibits NLRP3 inflammasome activation and reduces pyroptosis by enhancing PINK1/Parkinmediated mitophagy and attenuating ROS accumulation, which in turn ameliorates TS.