文献类型：期刊文献

中文题名：脾气虚大鼠骨骼肌组织Ca^(2+)/CaM信号通路关键分子动态表达规律

英文题名：Expression changes of Ca^(2+)/ CaM signaling pathways key factors in skeletal muscle tissue of rats with spleen-qi deficiency

作者：段永强[1];梁玉杰[2];成映霞[2];杜娟[1];杨晓轶[1];程卫东[3];刘靓[2];高建德[1];安耀荣[2];王燕[2]

第一作者：段永强

机构：[1]甘肃中医学院甘肃省中药药理与毒理学重点实验室;[2]甘肃中医学院甘肃省中药新产品创制重点实验室;[3]兰州大学基础医学院中西医结合研究所

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2015

卷号：0

期号：15

起止页码：4127

中文期刊名：中国老年学杂志

外文期刊名：Chinese Journal of Gerontology

收录：北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金项目(81160420);甘肃省自然科学基金项目(1010RJZA148);甘肃省教育厅基金项目(1006-05)

语种：中文

中文关键词：脾气虚证;Ca2+/Ca;M信号通路;骨骼肌

外文关键词：Spleen-qi deficiency;Ca2 +/ Ca M signaling pathways;Skeletal muscle

摘要：目的观察脾气虚大鼠骨骼肌组织Ca2+/钙调蛋白(Ca M)信号通路中关键分子〔Ca2+〕i以及Ca M、钙调蛋白激酶(Ca MK)Ⅱ、pCa MKⅡ蛋白表达水平的变化。方法受试动物随机分为正常对照组,脾虚模型7、14、21 d组,每组12只。除正常对照组外,其余受试动物采用复合法(苦寒破气法、游泳力竭法及饥饱失常法)成功建立脾气虚证大鼠模型,在观测各组大鼠一般生存状态、胃肠转运功能和骨骼肌组织ATP酶活性的基础上,采用激光共聚焦技术检测骨骼肌组织细胞内〔Ca2+〕i浓度,蛋白免疫印迹技术检测骨骼肌组织Ca M、Ca MKⅡ和p-Ca MKⅡ的表达变化。结果与空白组比较,脾气虚大鼠随着造模时间的延长,胃残留率升高而小肠推进率下降(P<0.01);骨骼肌组织Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性均降低(P<0.05,P<0.01);骨骼肌组织〔Ca2+〕i浓度和Ca M、Ca MKⅡ、p-Ca MKⅡ蛋白表达量显著降低(P<0.01);且脾虚模型7 d、14 d、21 d组之间比较,以脾虚21 d组变化更为显著。结论脾虚大鼠骨骼肌组织Ca2+/Ca M信号通路关键分子Ca M、Ca MKⅡ和p-Ca MKⅡ蛋白以低表达为主。
Objective To investigate the expression changes of Ca2 +/ Ca M signaling pathways key factors〔Ca2 +〕i,Ca M,Ca MKⅡand p-Ca MKⅡ in skeletal muscle tissue of rats with spleen-qi deficiency.Methods The rats were randomly divided into normal control,spleen-qi deficient model groups( observed on 7 d,14 d and 21 d),12 rats in each.The spleen-qi deficient model rats were made by rhubarb,exhaustive and hungry method,then general existence,gastric remnant rate,intestinal propulsion rates,and ATPase activity related to metabolism were evaluated.Also,confocal laser technology was used to test cellular〔Ca2 +〕i concentration and Western blot was used to test Ca M,Ca MKⅡ,p-Ca MKⅡexpressions in skeletal muscle of spleen asthenia rats.Results Compared with that of normal group,gastric remnant rate was increased and small intestinal propulsion rates were decreased( P < 0.01),the activities of Na+-K+-ATPase and Ca2 +-Mg2 +-ATPase were decreased significantly( P < 0.01,P < 0.05),and the expressions of Ca M,Ca MKⅡ,p-Ca MKⅡin skeletal muscle tissue were decreased,these differences were obvious on spleen-qi deficient model 21 d group( P < 0.01).Conclusions The 〔Ca2 +〕i concentrations,Ca M,Ca MKⅡand p-Ca MKⅡabnormaly decreased might be one of the pathological mechanisms of spleen-deficiency.