文献类型：期刊文献

中文题名：壳寡糖及酰化壳寡糖与钕(Ⅲ)配合物的合成及其与牛血清白蛋白相互作用的对比研究

英文题名：Synthesis of chitooligosaccharide and succinic-chitooligosaccharide with NdCl_3 and interaction with bovine serum albumin

作者：李小芳[1];冯小强[1];杨声[2]

第一作者：李小芳

机构：[1]天水师范学院化学工程与技术学院,甘肃天水741001;[2]定西师范高等专科学校,甘肃定西743000

第一机构：天水师范学院化学工程与技术学院,甘肃天水741001

年份：2015

卷号：27

期号：9

起止页码：1253

中文期刊名：化学研究与应用

外文期刊名：Chemical Research and Application

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：天水师院"青蓝"人才工程项目资助

语种：中文

中文关键词：壳寡糖;丁二酰化壳寡糖;钕(Ⅲ);牛血清白蛋白;相互作用

外文关键词：chitooligosaccharidesuccinic-chitooligosaccharide, Nd （Ⅲ） ; bovine serum albumin ; interaction

摘要：用壳寡糖及酰化壳寡糖与氯化钕反应,合成了壳寡糖-钕和酰化壳寡糖-钕配合物,利用红外光谱(IR)、紫外光谱(UV)手段对其结构进行了表征。在模拟生理条件下,本文采用紫外光谱和荧光光谱研究了两种配合物与牛血清白蛋白(BSA)的相互作用,计算了配合物与BSA的结合常数、结合位点数。荧光光谱结果表明,配合物均可有规律地猝灭BSA的内源荧光,猝灭方式为静态猝灭,壳寡糖-钕和酰化壳寡糖-钕分别与BSA的结合常数为1.33×104L·mol-1和6.95×104L·mol-1,结合位点数为1.05和1.3,说明配合物与BSA均具有较强的结合作用,能够被BSA储存和运输,并且酰化壳寡糖-钕与BSA的结合能力强于壳寡糖-钕。最后采用紫外光谱法对其作用机理进一步确认。因此,酰化壳寡糖-钕可以被BSA存储和运输,有望成为蛋白质荧光探针。
The coordination complexes of chitooligosaccharide with Nd^3＋（ CS-Nd） and succinic-chitooligosaccharide with Nd^3＋（ BCS-Nd）were prepared,which were synthesized by the reaction of chitooligosaccharide and succinic-chitooligosaccharide with NdCl3. The coordination complexes were characterized by infra-red spectrum and ultraviolet absorption spectrum,respectively. Moreover,the in-teraction between complexes and bovine serum albumins（ BSA） were studied under simulated physiological conditions by ultraviolet and fluorescence spectrum. Fluorescence experiment studies indicated that the reaction between the complexes and BSA was a static quenching procedure. The binding constant and binding sites are calculated for BSA with CS-Nd were 1. 33×10^4L·mol^-1and1. 05, BSA with BCS-Nd were 6. 95×10^4L·mol^-1and 1. 30,respectively. There was a strong binding reaction between complexes and BSA, and the rare earth complexes could be transported by BSA. The absorption intensity of BSA increased with the increasing of the com-plexes concentration,and shifted to lower wavelength of the maximum absorption peak with a blue. Therefore,BCS-Nd was expected to become a protein fluorescence probe.