文献类型：期刊文献

中文题名：基于正交实验法优化从栀子黄色素中制备藏红花酸的工艺

英文题名：Optimization of preparation process of crocetin from gardenia yellow pigment by orthogonal experiment

作者：阳志强[1];乌兰[1];李茂星[1,2,3,4];李晓琳[2,3];韩天翔[1];王维刚[2,3,4];王芃[2,3,4]

第一作者：阳志强

机构：[1]西北民族大学化工学院,兰州730030;[2]联勤保障部队第九四〇医院临床药学科;[3]甘肃省高原药学行业技术中心,兰州730050;[4]甘肃中医药大学药学院,兰州730000

第一机构：西北民族大学化工学院,兰州730030

年份：2022

卷号：34

期号：1

起止页码：76

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;CSCD:【CSCD2021_2022】；

基金：甘肃省重点人才项目(2021-0623-RCC-0038);中央高校基本科研业务费专项资金(31920200002);后勤科研项目(GLB21J036);拔尖培育项目(2021yxky001)。

语种：中文

中文关键词：藏红花酸;栀子黄色素;碱水解;高效液相色谱法;正交实验

外文关键词：crocetin;gardenia yellow pigment;alkaline hydrolysis;high performance liquid chromatography;orthogonal test

摘要：基于正交实验法,优化从栀子黄色素中提取制备藏红花酸的碱水解工艺,以期可以简单高效地获得高纯度藏红花酸。建立高效液相色谱法(HPLC)测定藏红花酸含量,以藏红花酸的含量和得率为考察指标,采用正交实验法考察碱水解工艺中的料液比、NaOH浓度、水解温度和水解时间对产品中藏红花酸含量和得率的影响。确定栀子黄色素碱水解的最佳条件:料液比1∶6 g/mL、NaOH浓度3 mol/L、水解温度55℃、水解时间60 min。在该条件下制备获得的藏红花酸得率可达15.33%±1.25%;含量可达到97.24%±0.78%。优化后方法步骤简单易行,绿色无污染,一步制得高纯度藏红花酸,适用于工业化生产。
To simply and efficiently prepare high-purity crocetin from gardenia yellow pigment,the alkaline hydrolysis process was optimized using an orthogonal experimental design.High-performance liquid chromatography(HPLC)was established for the determination of crocetin in the samples.The evaluation index was based on the purity and yield of crocetin.The solid-liquid ratio,NaOH concentration,hydrolysis temperature,and hydrolysis time were investigated.The optimal conditions for alkaline hydrolysis of gardenia yellow pigment were obtained as follows:solid-liquid ratio 1∶6 g/mL,NaOH concentration 3 mol/L,hydrolysis temperature 55°C,and hydrolysis time 60 min.Under this condition,the crocetin yield was 15.33%±1.25%and a content of 97.24%±0.78%.Finally,high-purity crocetin was prepared from the gardenia yellow pigment in a single step.This method is simple,environmentally friendly,and suitable for industrial application.