文献类型：期刊文献

中文题名：模拟高原低氧环境下大鼠HPA轴的应激性变化及脑组织水肿病理学观察

英文题名：Rat hyperirritable change of HPA axis and pathological observation of cerebral oadema in simu- lated environment of high altitude and hypoxia

作者：罗晓红[1];郭文静[2];牛小娟[1];牛廷献[3];冯小明[3];郭晓宇[3]

第一作者：罗晓红

机构：[1]兰州军区兰州总医院内分泌科,甘肃兰州730050;[2]甘肃中医药大学,甘肃兰州730000;[3]兰州军区兰州总医院动物实验科,甘肃兰州730050

第一机构：兰州军区兰州总医院内分泌科,甘肃兰州730050

年份：2016

卷号：36

期号：5

起止页码：459

中文期刊名：中国急救医学

外文期刊名：Chinese Journal of Critical Care Medicine

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：全军医药卫生科研基金项目（CLZ14JB06）

语种：中文

中文关键词：高原低氧;高原脑水肿(HACE);下丘脑-垂体-肾上腺轴(HPA);血管内皮生长因子(VEGF)

外文关键词：Hypobaric hypoxia; High altitude cerebral oadema （HACE）; Hypothalamic- pituitary-adrenal axis （HPA）; Vascular endothelial growth factor （VEGF）

摘要：目的探讨不同海拔梯度低氧环境下下丘脑-垂体-肾上腺（HPA）轴、血管内皮生长因子（VEGF）分泌规律及与高原脑水肿的相关性。方法将96只大鼠随机分为四组，即海拔1500m、3500m、5000m及6000m组，各组进一步分为缺氧12、24及48h，每组8只，共12组。1500m组置于我院动物实验科（1500m，兰州海拔）饲养，各缺氧组大鼠分别置于模拟不同海拔低压氧舱内，分别放置12、24及48h后取出，经心脏采集血液后处死；分离脑组织，用干湿质量法测定大鼠脑含水量；HE染色观察大鼠脑组织改变；ELISA法测定血清促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、糖皮质激素（GC）及VEGF含量；RT—PCR法测定缺氧诱导因子-1（HIF-1）mRNA、VEGF mRNA。结果与平原对照组比较，大鼠体质量减轻；高原缺氧模型组大鼠脑组织出现不同程度水肿，大鼠脑含水量增加，HPA轴分泌的CRH、ACTH、GC激素及VEGF含量随海拔高度上升而升高，差异有统计学意义（P〈0．05）；HIF—1 mRNA、VEGF mRNA随海拔高度升高呈上升趋势（P〈0.05）。结论随着海拔梯度的升高，HPA轴功能应激性调节增强，VEGF含量亦升高，并与脑组织水肿密切相关，提示HPA轴的应激调节机制在高原脑水肿的发病及预测中起到重要的作用。
Objective To explore the regulation hormone secretion of HPA axis, vascular endothelial growth factor in different hypoxic conditions and the correlation of high altitude cerebral oadema. Methods Ninety-six Wistar rats were randomly divided into four groups, 1500 m （control）, 3500 m, 5000 m and 6000 m groups; each group was further divided into hypoxia 12 h, 24 h, 48 h subgroups, to- tally 12 subgroups with 8 rats in each subgroup. The control rats were put in department of animal experi- ment （Lanzhou, at an altitude of 1500 m）; the hypoxia groups were put at different altitudes in hypobaric chamber. After hypoxia exposure for 12 h, 24 h, 48 h, heart blood was collected and the animals were then decapitated; brain tissue was collected to determine dry-wet proportion and histopathologic changes were observed with HE staining. The content of CRH, ACTH, GC, VEGF in serum were determined by ELISA. Real-time PCR was used to detect the expression of HIF- 1 and VEGF mRNA. Results Com- pared with control group, the rats weight decreased and the hypoxia groups showed obvious brain edema; the water content of rat brain tissue increased; the three main indicating hormones of HPA axis （CRH, ACTH, CORT） and VEGF increased with the increasing of altitude; there were statistical significances （P 〈 0.05）. The expression of HIF-1 mRNA, VEGF mRNA also increased with the increasing of altitude with statistical significances （P 〈 0.05）. Conclusion With the increasing of altitude, HPA axis stress reaction is abnormally enhanced; increased VEGF level by high altitude is closely related to high ahitude cerebral adema. HPA axis may play an important regulating role in the high altitude cerebral oadema invasion and prediction.