文献类型：期刊文献

英文题名：Effect of the tyrosinase inhibitor (S)-N-trans-feruloyloctopamine from garlic skin on tyrosinase gene expression and melanine accumulation in melanoma cells

作者：Wu, Yan[1];Wu, Zheng-Rong[2];Chen, Peng[2];Yang-Li[2];Deng, Wan-Rong[1];Wang, You-Quan[2];Li, Hong-Yu[2]

第一作者：吴艳;武燕

通信作者：Li, HY[1]

机构：[1]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Sch Pharmaceut, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharmaceut, 222 Tian Shui South Rd, Lanzhou 730000, Peoples R China.

年份：2015

卷号：25

期号：7

起止页码：1476

外文期刊名：BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000351688700023)】；

基金：This study was supported by the Fundamental Research Funds for the Central Universities of China in 2014 (lzujbky-2014-150), Gansu Province Science Foundation for Distinguished Young Scholars (Grant No. 1308RJDA014), Longyuan Support Project for Young Creative Talents (Grant No. GANZUTONGZI [2014] no.4), Technology Program of Gansu Province (Grant No. 1207TCYA034) and Technology Program of Lanzhou City (Grant No. 2013-4-115).

语种：英文

外文关键词：(S)-N-trans-Feruloyloctopamine; Cytotoxicity; Anti-melanogenic effect; Tyrosinase expression

摘要：In our searching for novel tyrosinase inhibitors from natural sources, (S)-N-trans-feruloyloctopamine isolated from garlic skin was found to be a potential mushroom tyrosinase inhibitor. Here, we examined the effects of the potential tyrosinase inhibitor in B16F10 cells on intracellular melanin contents, cytotoxicity, and the signaling mechanism involved in the expression of tyrosinase. The results showed the inhibitor displayed little or no cytotoxicity at all concentrations examined and decreased the relative melanin contents in a dose-dependent manner in the alpha-MSH-stimulated B16F10 cells. Real-time PCR and Western blot analysis showed that it inhibits melanogenesis signaling by down-regulates mRNA and protein expression levels of tyrosinase, which leads to a lower melanin contents. These results suggested that (S)-N-trans-feruloyloctopamine was an ideal tyrosinase inhibitor, and could be used in food and medical industry. (C) 2015 Elsevier Ltd. All rights reserved.