文献类型：期刊文献

英文题名：RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs

作者：Zhang, Liying[1];Zhou, Ting[1];Zhang, Yiming[1];Wang, Lei[2];Li, Yangyang[1];Zhou, Gucheng[1];Zhou, Heng[3];Niu, Fan[4];Liu, Zhiwei[1];Miao, Zhiming[1];Liu, Yongqi[1,5,6]

第一作者：张利英

通信作者：Liu, YQ[1];Liu, YQ[2]

机构：[1]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou, Peoples R China;[2]Chinese Acad Chinese Med Sci, Beijing, Peoples R China;[3]Yangzhou Univ, Sch Publ Hlth, Yangzhou, Peoples R China;[4]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Peoples R China;[5]Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou, Peoples R China;[6]Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：20

期号：2

起止页码：516

外文期刊名：PHARMACOGNOSY MAGAZINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001144894000001)】；

基金：We acknowledge Gansu University of Chinese Medicine, Provincial Level key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities and Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level for providing support and assistance for this article.

语种：英文

外文关键词：Radiation; bystander effect; astragalus polysaccharide; methylation; deterioration risk

摘要：Background: Ionizing radiation can induce bystander effects (RIBE), and astragalus polysaccharides (APS) have a protective effect against RIBE. It has been proved that RIBE occurred and APS played the inhibitory effects on the RIBE when radiation was carried out only one time. However, whether RIBE happened by multiple irradiations and the effects of APS on the RIBE are unclear. This study aimed to investigate whether APS suppress RIBE damage induced by multiple irradiations. Materials and Methods: A549 cells were irradiated with 2 Gy X-rays to obtain a conditioned medium. Bone mesenchymal stem cells (BMSCs) was incubated with the conditioned medium 5 times every 3 days or APS. Cell proliferation was detected by CCK-8 and colony formation assay, and genomic instability and DNA damages were detected by the micronucleus and immunofluorescence assay. The protein associated with methylation and cancer-associated proteins in BMSCs were detected by western blot. Results: After five stimulations with a conditioned medium, the proliferative capacity of BMSCs decreased; APS pre-intervention promoted the proliferation of BMSCs. And conditional medium intervention increased the micronucleus rate and a number of 53BP1 foci; APS pre-intervention reduced the micronucleus rate and number of 53BP1 foci. In addition, the conditional medium intervention reduced the expression of methylation-related proteins and increased the expression of cancer-related proteins, while APS pre-intervention reversed this trend. Conclusion: Irradiated A549 conditioned medium can induce RIBE of BMSCs, which might be related to methylation and cancer-associated proteins and APS may block RIBE by multiple irradiations in BMSCs.