文献类型：期刊文献

中文题名：大黄总多酚、总蒽醌含量测定及体外抗氧化作用的谱效关系研究

英文题名：Study on content of total polyphenols and total anthraquinones in rhubarb and its spectrum-effect relationship of anti-oxidation in vitro

作者：李东辉[1,2];吴红伟[1,2];李国峰[1,2];杨新荣[1,2];李咸慰[1,2];宋沁洁[1,2];李阳[1,2];李越峰[1,2]

第一作者：李东辉

机构：[1]甘肃中医药大学;[2]甘肃省中药制药工艺工程研究中心,兰州730000

第一机构：甘肃中医药大学

年份：2022

卷号：34

期号：4

起止页码：541

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;CSCD:【CSCD2021_2022】；

基金：甘肃省中药制药工艺工程研究中心开放课题(ZYGY202003);甘肃省高等学校科学研究项目(2015B-074);国家自然科学基金(82160750);甘肃省教育厅产业支撑计划(2021CYZC-21)。

语种：中文

中文关键词：大黄;总多酚;总蒽醌;指纹图谱;抗氧化作用

外文关键词：rhubarb;total polyphenols;total anthraquinones;fingerprinting;antioxidant effect

摘要：本文旨在研究20批掌叶大黄饮片总蒽醌含量,总多酚含量,初步分析体外抗氧化活性的谱效关系。采用高效液相测定大黄的总蒽醌含量,采用紫外分光光度计测定总多酚含量,利用DPPH法和ABTS法对大黄的体外抗氧化活性进行评价,并利用HPLC指纹图谱,以共有峰为研究对象,采用聚类分析、主成分分析,偏最小二乘分析,灰色关联分析及Pearson相关系数法对其抗氧化活性进行谱效分析,并借助反应前后色谱峰峰面积变化作为谱效分析的补充。结果表明,总蒽醌含量1.06±0.08~4.82±0.12 mg/g,总多酚含量在4.921±0.21~10.02±0.11 mg/g,聚类分析及主成分分析表明20批大黄样品可分为4类,即陇西县、临洮县为一类,宕昌县、礼县为一类,岷县、漳县为一类,渭源县为一类。共有峰7(芦荟大黄素-8-O-葡萄糖苷)、1、10(大黄酸)、2、9(芦荟大黄素)具有清除DPPH自由基活性的作用,共有峰7、1、10、3、8、12(大黄素)及14(大黄素甲醚)具有清除ABTS自由基活性的作用,这与反应前后共有峰峰面积变化结果基本一致。相关性分析表明共有峰10、7与清除DPPH自由基活性正相关,总蒽醌与清除DPPH自由基活性负相关。共有峰2、11与清除ABTS自由基活性负相关,总多酚与清除ABTS自由基活性呈显著正相关。该研究为大黄抗氧化活性药效物质成分的筛选及区分不同产地大黄提供理论依据。
This research aims to study the total anthraquinone content and total polyphenol content of 20 batches of rhubarb palm decoction pieces,and to preliminarily analyze the spectrum-effect relationship of antioxidant activity in vitro.The total anthraquinone content of rhubarb was determined by HPLC,the total polyphenol content was determined by UV spectrophotometry,DPPH and ABTS methods were used to evaluate the antioxidant activity of rhubarb in vitro.HPLC fingerprint was used to take the common peak as the research object,and cluster analysis,principal component analysis,partial least square analysis,gray correlation analysis and Pearson correlation coefficient method were used to analyze the antioxidant activity.The change of chromatographic peak area before and after the reaction was used as the supplement of spectral analysis.The results showed that the total anthraquinone content was 1.06 ± 0.08-4.82 ± 0.12 mg/g,and the total polyphenol content was 4.921 ± 0.21-10.02 ± 0.11 mg/g.Cluster analysis and principal component analysis showed that 20 batches of rhubarb samples could be divided into 4 class,namely Longxi County and Lintao County are the first category,Dangchang County and Li County are the first category,Min County and Zhang County are the first category,and Weiyuan County is the first category.Common peaks 7(aloe emodin-8-O-glucoside),1,10(emodin),2,9(aloe emodin) had scavenging effect on DPPH free radical activity,and common peaks 7,1,10,3,8,12(emodin) and 14(emodin methyl ether) had scavenging effect on ABTS free radical activity.This is basically consistent with the change of common peak area before and after the reaction.Correlation analysis shows that the common peaks 10 and 7 are positively correlated with the activity of scavenging DPPH free radicals,and total anthraquinones are negatively correlated with the activity of scavenging DPPH free radicals.Common peaks 2 and 11 are negatively correlated with the activity of scavenging ABTS free radicals,and total polyphenols are significantly positively correlated with the activity of scavenging ABTS free radicals.This research provides a theoretical basis for the screening of antioxidative active ingredients of rhubarb and the differentiation of rhubarb from different origins.