文献类型：期刊文献

中文题名：基于Rho/Rock通路探讨当归红芪多糖对辐射诱导大鼠H9C2心肌细胞的作用机制

英文题名：Study on Mechanism of Angelica and Hedysari Polysaccharide on Radiation-induced H9C2 Cardiac Cells Based on Rho/Rock Pathway

作者：邢喜平[1];任春贞[1,2];蒋虎刚[2];马承旭[2];刘凯[2];卢玉俊[1];李应东[2];赵信科[1]

第一作者：邢喜平

机构：[1]甘肃中医药大学附属医院,甘肃兰州730000;[2]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2021

卷号：48

期号：7

起止页码：228

中文期刊名：辽宁中医杂志

外文期刊名：Liaoning Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金(81860786,81760798);中医药循证能力建设项目(2019XZZX-XXG002)

语种：中文

中文关键词：当归红芪多糖;Rho/Rock通路;细胞周期;放射性心肌纤维化

外文关键词：Angelica Hedysari polysaccharide;Rho/ROCK pathway;cell cycle;radiation myocardial fibrosis

摘要：目的基于Ras同源基因(Rho)/Rho激酶信号通路对放射性心肌纤维化发病机制及当归红芪多糖的干预作用进行探索。方法首先建立放射剂量梯度,通过筛选放射性心肌损伤的最佳浓度,建立放射性心肌损伤模型。通过法舒地尔、不同浓度当归红芪多糖溶液进行干预,同时采用Western Blot、QT-PCR、流式细胞术3种实验手段检测Rho/Rock及其下游通路的活化、心肌细胞周期、心肌纤维化的改变。结果当归红芪多糖干预后Rho/Rock及其下游通路的活化明显抑制,细胞周期阻滞明减轻,心肌纤维化被逆转。结论当归红芪多糖可能通过抑制Rho/Rock及其下游通路的活化、减轻细胞周期阻滞等逆转放射性心肌纤维化。
Objective Based on Rho/ROCK pathway,to explore the pathogenesis of radiation-induced myocardial fibrosis and the intervention of Angelica Hedysari polysaccharide.Methods The radiation dose gradient was established so as to select the best concentration and establish the radiation myocardial injury model.Western blot,QT-PCR and flow cytometry were used to detect the activation of Rho/rock and its downstream pathway,myocardial cell cycle and myocardial fibrosis.Results The activation of Rho/rock and its downstream pathway were significantly inhibited,cell cycle arrest was reduced and myocardial fibrosis was reversed.Conclusion Angelica Hedysarum polysaccharide may reverse the radiation myocardial fibrosis by inhibiting the activation of Rho/rock and its downstream pathway,reducing cell cycle arrest and so on.