文献类型：期刊文献

英文题名：The protective effect and bioactive compounds of Astragalus membranaceus against neurodegenerative disorders via alleviating oxidative stress in Drosophila

作者：Dai, Yuting[1];Wang, Yixuan[1];Kang, Qian[2,3,4];Wu, Yifan[1];Liu, Yongqi[2,3,5];Su, Yun[2,3,4,5];Wang, Xinhua[1];Xiu, Minghui[1,5];He, Jianzheng[2,3,5]

第一作者：Dai, Yuting

通信作者：Xiu, MH[1];He, JZ[2];He, JZ[3]

机构：[1]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Prevent & Treatment Tradit Chinese Med Res Gansu, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Res & Expt Ctr, Lanzhou, Peoples R China;[5]Minist Educ, Key Lab Dunhuang Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Prevent & Treatment Tradit Chinese Med Res Gansu, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;

年份：2024

卷号：38

期号：13

外文期刊名：FASEB JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001247950900001)】；

基金：We are grateful to members of Xiu and He laboratory for useful comments on the manuscript.

语种：英文

外文关键词：antioxidant; Astragalus membranaceus; bioactive compounds; Drosophila melanogaster; neurodegenerative diseases

摘要：Oxidative stress is proposed as a regulatory element in various neurological disorders, which is involved in the progress of several neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Antioxidant drugs are widely used to alleviate neurodegenerative disorders. Astragalus membranaceus (Huangqi, AM) is a commonly used medicinal herb with a wide range of pharmacological effects. Here, the protective effect and mechanism of AM extract (AME) and its bioactive compounds against neurodegenerative disorders via alleviating oxidative stress were detected using adult Drosophila melanogaster. The drug safety was measured by development analysis; oxidative stress resistance ability was detected by survival rate under H2O2 environment; ROS level was detected by DHE staining and gstD1-GFP fluoresence assay; antioxidative abilitiy was represent by measuring antioxidant enzyme activity, antioxidative-related gene expression, and ATP and MFN2 levels. The neuroprotective effect was evaluated by lifespan and locomotion analysis in A beta 42 transgenic and Pink1(B9) mutants. AME dramatically increased the survival rates, improved the CAT activity, restored the decreased mRNA expressions of Sod1, Cat, and CncC under H2O2 stimulation, and ameliorated the neurobehavioral defects of the AD and PD. Thirteen small molecules in AM had antioxidant function, in which vanillic acid and daidzein had the most potent antioxidant effect. Vanillic acid and daidzein could increase the activities of SOD and CAT, GSH level, and the expressions of antioxidant genes. Vanillic acid could improve the levels of ATP and MFN2, and mRNA expressions of ND42 and SDHC to rescue mitochondrial dysfunction. Furthermore, vanillic acid ameliorated neurobehavioral defects of PD. Daidzein ameliorated neurobehavioral defect of A beta-induced AD mode. Taken together, AM plays a protective role in oxidative damage, thereby as a potential natural drug to treat neurodegenerative disorders.