文献类型：期刊文献

英文题名：Collagen/heparin sulfate scaffold combined with mesenchymal stem cells treatment for canines with spinal cord injury: A pilot feasibility study

作者：Deng, Wu-Sheng[1];Yang, Kun[2];Liang, Bing[3];Liu, Ying-Fu[4];Chen, Xu-Yi[3];Zhang, Sai[3]

第一作者：Deng, Wu-Sheng

通信作者：Chen, XY[1];Zhang, S[1]

机构：[1]Gansu Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Lanzhou, Gansu, Peoples R China;[2]First Affiliated Hosp, Hainan Med Coll, Haikou, Hainan, Peoples R China;[3]Pingjin Hosp Brain Ctr, Characterist Med Ctr Chinese Peoples Armed Police, Tianjin Key Lab Neurotrauma Repair, Tianjin 300162, Peoples R China;[4]Cang Zhou Nanobody Technol Innovat Ctr, Cangzhou, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Pingjin Hosp Brain Ctr, Characterist Med Ctr Chinese Peoples Armed Police, Tianjin Key Lab Neurotrauma Repair, Tianjin 300162, Peoples R China.

年份：2021

卷号：29

期号：2

外文期刊名：JOURNAL OF ORTHOPAEDIC SURGERY

收录：;Scopus(收录号：2-s2.0-85107141997);WOS:【SCI-EXPANDED(收录号:WOS:000711435600001)】；

语种：英文

外文关键词：canine; collagen; heparin sulfate scaffold; human mesenchymal stem cells; nerve regeneration; spinal cord injury

摘要：Background: Due to endogenous neuronal deficiency and glial scar formation, spinal cord injury (SCI) often leads to irreversible neurological loss. Accumulating evidence has shown that a suitable scaffold has important value for promoting nerve regeneration after SCI. Collagen/heparin sulfate scaffold (CHSS) has shown effect for guiding axonal regeneration and decreasing glial scar deposition after SCI. The current research aimed to evaluate the utility of the CHSSs adsorbed with mesenchymal stem cells (MSCs) on nerve regeneration, and functional recovery after acute complete SCI. Methods: CHSSs were prepared, and evaluated for biocompatibility. The CHSSs adsorbed with MSCs were transplanted into these canines with complete SCI. Results: We observed that MSCs had good biocompatibility with CHSSs. In complete transverse SCI models, the implantation of CHSS co-cultured with MSCs exhibited significant improvement in locomotion, motor evoked potential, magnetic resonance imaging, diffusion tensor imaging, and urodynamic parameters. Meanwhile, nerve fibers were markedly improved in the CHSS adsorbed with MSCs group. Moreover, we observed that the implantation of CHSS combined with MSCs modulated inflammatory cytokine levels. Conclusions: The results preliminarily demonstrated that the transplantation of MSCs on a CHSS could improve the recovery of motor function after SCI. Thus, implanting the MSCs-laden CHSS is a promising combinatorial therapy for treatment in acute SCI.