文献类型：期刊文献

中文题名：氯化钠浓度对黄芩幼苗生长情况及生理指标的影响

英文题名：Effect of NaCl Concentration on Seedling Growth and Physiological Indexes of Scutellaria baicalensis

作者：郭菊梅[1];陈红刚[2];曹瑞[2];杜弢[2]

第一作者：郭菊梅

机构：[1]陇西县种子管理站,甘肃陇西748100;[2]甘肃中医学院药学系,兰州730000

第一机构：陇西县种子管理站,甘肃陇西748100

年份：2014

卷号：25

期号：47

起止页码：4497

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省中药材产业科技攻关项目(No.GYC1205)

语种：中文

中文关键词：黄芩幼苗;盐胁迫;成活率;膜透性;丙二醛;叶绿素;类胡萝卜素

外文关键词：Scutellaria baicalensis seedling; Salt stress; Survival rate; Membrane permeability; MDA; Chlorophyll; Carot-enoid

摘要：目的：探讨氯化钠（NaCl）浓度对黄芩幼苗生长情况及生理指标的影响,为黄芩药材的大田生长提供依据。方法：采用盆栽方式,待黄芩幼苗长至8～11 cm时,用1/2 Hoagland营养液兑加不同剂量的NaCl,模拟盐胁迫环境,培养3 d后统计幼苗成活率,并采用紫外-可见分光光度法测定叶绿素和类胡萝卜素的含量,用硫代巴比妥酸法测定丙二醛的含量,用电导法定膜透性。结果：随着NaCl浓度的增大,黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均呈先升后降的趋势,丙二醛含量和相对电导率呈逐渐升高的趋势;其中,NaCl浓度为0.4%时,黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均达到峰值。结论：黄芩幼苗的临界耐盐浓度为0.4%,盐浓度〈0.4%时对黄芩幼苗的生长无明显影响,〉0.4%时盐害作用较严重。
OBJECTIVE：To investigate the effect of NaCl concentration on seedling growth and physiological indexes of Scutel- laria baicalensis, and to provide reference for the growth of S. baicalensis in large area. METHODS： Seedlings of S. baicalensis potted, 8-11 cm in height, were cultured in 1/2 Hoagland nutrient solution with different concentrations of NaC1. Under simulated salt stress condition, the survival rate of the seedlings was analyzed statistically after 3 days of culture. The contents of chlorophyll and carotenoid were determined by UV visible spectrophotometry, and the content of malonaldehyde was determined by thiobarbitu- ric acid method. The membrane permeability was determined by conductivity method. RESULTS： With the increase of NaCI concen- tration, the survival rate of seedling became raised firstly, and declined subsequently, however, the content of MDA and chloro- phyll, relative conductivity became raised continuously. The content of carotenoid became raised firstly, and declined subsequently, too. When NaCl concentration was 0.4%, survival rate and total content of chlorophyll reached its peak. CONCLUSIONS：The criti- cal salt-tolerant concentration for S. baicalensis seedling is 0.4%. When the concentration of NaCl is less than 0.4%, it has no sig- nificant effect on the growth of S. baicalensis seedling; when the concentration of NaC1 is more than 0.4%, serious salt injury have happened.