文献类型：期刊文献

英文题名：Astragalus saponin attenuates the expression of fibrosis-related molecules in irradiated cardiac fibroblasts

作者：Gu, Jing[1,2,3];Liu, Kai[2];Li, Hailong[2];Wang, Xiaogang[1];Yang, Kehu[1]

第一作者：顾静;Gu, Jing

通信作者：Yang, KH[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Evidence Based Med Ctr, Lanzhou 730000, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China;[3]Key Lab Evidence Based Med & Knowledge Translat G, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Evidence Based Med Ctr, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Evidence Based Med Ctr, Lanzhou 730000, Peoples R China.

年份：2014

卷号：46

期号：6

起止页码：492

外文期刊名：ACTA BIOCHIMICA ET BIOPHYSICA SINICA

收录：;Scopus(收录号：2-s2.0-84901666454);WOS:【SCI-EXPANDED(收录号:WOS:000337029500008)】；

基金：The work is supported by grants from the Natural Science Foundation of Gansu Province of China (No. 1310RJZA088), the Fundamental Research Funds for Gansu Universities of China (No. 2012-01), Scientific Research Projects of Health Care Industry of Gansu Province (No. GWGL2011-10), and Youth Foundation of Gansu University of Traditional Chinese Medicine (No. ZQ2011-15).

语种：英文

外文关键词：radiation-induced fibrosis damage; cardiac fibroblast; Astragalus saponin; TGF-beta 1

摘要：The main pathological change of radiation-induced heart disease is fibrosis. Emerging evidence has indicated that Astragalus membranaceus and its extractant, Astragalus saponin (AST), were used for treating fibrosis diseases. In the present study, the effects of AST on fibrosis damage induced by irradiation were determined. After being irradiated with 1 or 2-Gy X-rays, obvious changes of endoplasmic reticulum morphology were observed in cardiac fibroblasts (CFs), suggesting that its protein processing function was imbalanced, which indirectly indicated that fibrosis damage was caused by irradiating CFs. The expression levels of TGF-beta 1 and collagen I (Col-1) were increased at 48-h post-irradiation. Administration of 20 mu g/ml AST reduced the production of reactive oxygen species in irradiated CFs and decreased the expression of Col-1, TGF-beta 1, and p-Smad2/3. Polymerase chain reaction (PCR)-array analysis showed that there were similar to 30 genes which were mainly classified into extracellular matrix, remodeling enzymes, inflammatory cytokines/chemokines, and TGF-beta superfamily, were up-regulated after treatment with 1-Gy X-ray, whereas most of these genes were down-regulated when pretreated with 20 mu g/ml of AST. In addition, TIMP1 and Smad7 genes that were down-regulated after treatment with 1-Gy X-ray were up-regulated when pretreated with 20 mu g/ml of AST. In conclusion, radiation-induced fibrosis damage was observed at a cellular level. AST attenuated this fibrosis damage effect in irradiated CFs and this anti-fibrosis effect may be closely related to its antioxidant action. The involvement of fibrosis-related molecules in irradiated CFs was systematically demonstrated by a PCR array for the first time. AST reversed the expression of the majority of genes changed by irradiation, which further confirmed its anti-fibrosis effect.