文献类型：期刊文献

中文题名：丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究

英文题名：Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods

作者：李小芳[1];冯小强[1];杨声[2];王霞[1]

第一作者：李小芳

通信作者：Feng, X.-Q.

机构：[1]天水师范学院生命科学与化学学院,甘肃天水741001;[2]定西师范高等专科学校,甘肃定西743000

第一机构：天水师范学院生命科学与化学学院,甘肃天水741001

通信机构：[1]College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China

年份：2013

卷号：34

期号：12

起止页码：1662

中文期刊名：发光学报

外文期刊名：Chinese Journal of Luminescence

收录：CSTPCD;;EI(收录号：20140517252847);Scopus(收录号：2-s2.0-84892905835);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：天水师范学院"青蓝"人才工程资助项目

语种：中文

中文关键词：丁二酰化壳寡糖;稀土;配合物;鲱鱼精DNA

外文关键词：succinic-oligochitosan;rare earth;complexes;herring sperm DNA

摘要：采用紫外光谱和循环伏安法,研究了丁二酰化壳寡糖稀土配合物(BCS-La、BCS-Nd)与鲱鱼精DNA之间的作用方式。BCS-La、BCS-Nd的存在导致Fe(CN)3-/4-6探针分子峰电流下降,式量电位正移,表明BCSLa、BCS-Nd和探针分子与DNA之间存在竞争性作用;BCS-La和BCS-Nd分子都是通过插入方式与DNA相互作用。在一定的扫描速率范围内(0.01～0.2 V/s),在BCS-La或BCS-Nd参与的条件下,Fe(CN)3-/4-6在Au/DNA电极上的反应受吸附控制。BCS-La和BCS-Nd分别使得鲱鱼精DNA的特征峰产生明显的减色效应,最大吸收峰峰位红移,进一步表明BCS-La和BCS-Nd分别以插入方式与鲱鱼精DNA发生相互作用,导致DNA分子的构象变化。BCS-La与DNA的结合比为:n(BCS-La)∶n(DNA)=2∶1;n(BCS-Nd)∶n(DNA)=6∶1。
The interaction between succinic-oligochitosan-rare earth complexes( BCS-La and BCSNd) and herring sperm DNA was investigated by cyclic voltammetry and UV-Vis spectra. The results indicated as follows. Firstly,the peak current of probe molecule Fe( CN)3/46 at Au / DNA electrodes obviously decreased and the peak potential shifted positively because of the existences of complexes. All above these showed that there was a competitive effect between complexes and probe molecule with herring sperm DNA,which revealed that a binding mode intercalation was interacted between complexes and herring sperm DNA. The reaction of Fe( CN)3/46 at Au / DNA electrodes was controlled by adsorption mechanism in the range of scan rate from 0. 01 V / s to 0. 2 V / s. Secondly,the absorption intensity of herring sperm DNA decreased with the adding of complexes,and the maximum absorption peak had a red shift,which further illustrated that a binding mode intercalation was interacted between complexes and herring sperm DNA,thus led to the change of DNA conformation. The combining ratio of n( BCS-La) and n( BCS-Nd) with DNA was 2∶ 1 and 6∶ 1,respectively.