文献类型：期刊文献

中文题名：秦艽不同配伍对风寒湿痹类风湿关节炎模型大鼠关节保护作用及其机制

英文题名：Protective effect of different compatibility of Gentianae Macrophyllae Radix on joints of rheumatoid arthritis rats with wind-cold-dampness arthralgia and its mechanism

作者：苏杉[1];王蓉[1];刘飞[1];马腾茂[1];曹林忠[2];高慧琴[1]

第一作者：苏杉

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃中医药大学中医临床学院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：34

期号：21

起止页码：2542

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区基金资助项目(81360648)

语种：中文

中文关键词：秦艽;风寒湿痹;类风湿关节炎;基质金属蛋白酶3;基质金属蛋白酶组织抑制剂1

外文关键词：Gentianae Macrophyllae Radix;wind - cold - dampness arthralgia;rheumatoid arthritis;matrix metallopro-teinase - 3;tissue inhibitor of matrix metalloproteinase - 1

摘要：目的基于免疫组化技术观察秦艽不同配伍对风寒湿痹类风湿关节炎(RA)大鼠踝关节基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶组织抑制剂-1(TIMP-1)的影响。方法按随机数字表法将大鼠分为8组:空白组、Ⅱ型胶原模型组、风寒湿病证模型组、对照组、秦艽组、秦艽-威灵仙组、秦艽-桑寄生组、秦艽-防己组,每组10只。造模结束后,各中药组按25 g·kg^(-1)给予相应药液,对照组按6 mg·kg^(-1)给予雷公藤多苷混悬液,其余组给予等量0. 9%NaCl,1天1次,连续21 d。以免疫组化检测踝关节MMP-3、TIMP-1的表达。结果空白组、Ⅱ型胶原模型组、风寒湿病证模型组、对照组、秦艽组、秦艽-威灵仙组、秦艽-桑寄生组、秦艽-防己组的MMP-3光密度值分别为(3. 71±0. 80)×10~3,(9. 63±0. 18)×10~3,(11. 66±0. 62)×10~3,(6. 06±0. 94)×10~3,(7. 67±0. 46)×10~3,(5. 84±0. 39)×10~3,(7. 38±0. 37)×10~3,(8. 49±0. 37)×10~3;这8组的TIMP-1光密度值分别为(26. 62±3. 35)×10~3,(5. 64±1. 03)×10~3,(3. 94±0. 47)×10~3,(21. 77±2. 48)×10~3,(13. 80±3. 26)×10~3,(19. 77±2. 08)×10~3,(13. 48±2. 38)×10~3,(9. 14±1. 19)×10~3,Ⅱ型胶原模型组和病证模型组与空白组比较,MMP-3与TIMP-1表达的差异均有统计学意义(均P <0. 01);各给药组与病证模型组比较,MMP-3与TIMP-1表达差异均有统计学意义(均P <0. 01)。结论对于风寒湿痹型RA,平温相配的治疗效果优于平平相配、平寒相配及单味秦艽,实验结果与中医"寒者热之"的治则基本相符。
Objective To explore the influence of different compatibility of Gentianae Macrophyllae Radix（ Qinjiao） on the expression of matrix metalloproteinase-3（ MMP-3）,tissue inhibitor of metalloproteinase-1（ TIMP-1） in ankle joint of rheumatoid arthritis（ RA） model rats. Methods SD rats were randomly divided into 8 groups： blank group,type Ⅱ collagen model group,wind-cold-dampness syndrome model group,control group,Qinjiao group,Qinjiao-Weilingxian group,Qinjiao-Sangjisheng group,Qinjiao-Fangji group. Type Ⅱ collagen model group was injected with type Ⅱ collagen emulsion only,except for the blank group; the other groups were injected with type Ⅱ collagenemulsion and given wind-cold-dampness stimulus to prepare the wind-cold-dampness RA rat model. After the establishment of the model,the blank group,the 2 model groups were given normal saline; the control group was given 6 mg·kg^-1 tripterygium glycosides suspension; Qinjiao group,Qinjiao-Weilingxian group,Qinjiao-Sangjisheng group,Qinjiao-Fangji Group were given respectively 25 g·kg^-1 corresponding medicinal liquid. The expression of MMP-3 and TIMP-1 in ankle joint was detected by immunohistochemistry. Results The optical density values of MMP-3 in blank group,type Ⅱ collagen model group,wind-cold-dampness syndrome model group,control group,Qinjiao group,Qinjiao-Weilingxian group,Qinjiao-Sangjisheng group,Qinjiao-Fangji group were respectively（ 3. 71 ± 0. 80） × 10^3,（ 9. 63 ± 0. 18） × 10^3,（ 11. 66 ± 0. 62） × 10^3,（ 6. 06 ± 0. 94） × 10^3,（ 7. 67 ± 0. 46） × 10^3,（ 5. 84 ± 0. 39） × 10^3,（ 7. 38 ± 0. 37） × 10^3,（ 8. 49 ± 0. 37） × 10^3; TIMP-1 optical density values in the 8 groups were（ 26. 62 ± 3. 35） × 10^3,（ 5. 64 ± 1. 03） × 10^3,（ 3. 94 ± 0. 47） × 10^3,（ 21. 77 ± 2. 48） × 10^3,（ 13. 80 ± 3. 26） × 10^3,（ 19. 77 ± 2. 08） × 10^3,（ 13. 48 ± 2. 38） × 10^3,（ 9. 14 ± 1. 19） × 10^3. Comparison between the 2 model groups and blank group,the difference had statistical significance（ all P 0. 01）; the difference between the wind-cold-dampness syndrome model group and the each administration groups had statistical significance（ all P 0. 01）. Conclusion For rheumatoid arthritis of wind-cold-dampness arthralgia type,the therapeutic effect of the combination of mild and warm is better than that of the combination of mild and the combination of cold and mild and single-taste Qinjiao. The experimental results are basically consistent with the therapeutic principle of＂treating cold diseases should use hot medicine＂.