详细信息

Total polysaccharides of adlay bran (Coix lachryma-jobi L.) improve TNF-α induced epithelial barrier dysfunction in Caco-2 cells via inhibition of the inflammatory response  ( SCI-EXPANDED收录)   被引量:43

文献类型:期刊文献

英文题名:Total polysaccharides of adlay bran (Coix lachryma-jobi L.) improve TNF-α induced epithelial barrier dysfunction in Caco-2 cells via inhibition of the inflammatory response

作者:Li, Yanlong[1];Tian, Xudong[1];Li, Shengcai[1];Chang, Lijun[2];Sun, Ping[1];Lu, Yubei[1];Yu, Xiaoyan[1];Chen, Shiwang[1];Wu, Zhengquan[1];Xu, Zhong[1];Kang, Wanrong[3]

第一作者:Li, Yanlong

通信作者:Chang, LJ[1]

机构:[1]Gansu Hosp TCM, Dept spleen & stomach Dis, Lanzhou 730050, Gansu, Peoples R China;[2]Gansu Prov Ctr Dis Control & Prevent, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ TCM, SPF Anim Lab, Lanzhou 730030, Gansu, Peoples R China

第一机构:Gansu Hosp TCM, Dept spleen & stomach Dis, Lanzhou 730050, Gansu, Peoples R China

通信机构:[1]corresponding author), Gansu Prov Ctr Dis Control & Prevent, Lanzhou 730000, Gansu, Peoples R China.

年份:2019

卷号:10

期号:5

起止页码:2906

外文期刊名:FOOD & FUNCTION

收录:;WOS:【SCI-EXPANDED(收录号:WOS:000469145900055)】;

基金:The present work was financially supported by the Science-Technology Foundation for Young Scientists of Gansu Province, China (Grant No. 18JR3RA072).

语种:英文

摘要:Dysfunction of the intestinal epithelial barrier plays an important role in the pathogenesis of several intestinal diseases, including celiac disease, inflammatory bowel disease, and irritable bowel syndrome. The present research was carried out to investigate the protective effect of total polysaccharides of adlay bran (TPA) on TNF--evoked epithelial barrier dysfunction in Caco-2 cells. Caco-2 cells were treated with or without TPA in the absence or presence of TNF-, and transepithelial electrical resistance (TEER) and Phenol Red flux were assayed to evaluate the intestinal epithelial barrier function. The results indicated that TPA suppressed the TNF--induced release of pro-inflammatory factors. Furthermore, TPA obviously assuaged both the increased paracellular permeability and the decrease of TEER in TNF--challenged Caco-2 cells. Furthermore, TPA obviously assuaged TNF--evoked up-regulation of IL-8 and IL-6 expression, down-regulation of occludin and ZO-3 expression, and markedly suppressed the activation and protein expression of NF-B p65. Our results indicated that TPA assuages the TNF--evoked dysfunction of the intestinal epithelial barrier by inhibiting the NF-B p65-mediated inflammatory response.

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