文献类型：期刊文献

英文题名：Protective effect of quercetin on lung epithelial cell bystander-effect in the conditioned medium model of lung cancer metastasis induced by radiation

作者：Hong, Tao[1];Li, Juan[1];Su, Miao[1];Du, Shuanggui[1];Liang, Jianqing[1];Zhang, Yi[1];Zhang, Yiming[2];Miao, Zhiming[2];Ma, Tianxing[1];Li, Jintian[1]

第一作者：Hong, Tao

通信作者：Li, JT[1]

机构：[1]Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：69

期号：3

起止页码：156

外文期刊名：CELLULAR AND MOLECULAR BIOLOGY

收录：;Scopus(收录号：2-s2.0-85163047315);WOS:【SCI-EXPANDED(收录号:WOS:001017190100023)】；

基金：& nbsp;This study was supported by the National Natural Science Foundation of China [Nos. 81460695 and 82160872] and the Gansu Province "Double First Class" Key Scientific Research Project [No. 30140407] .

语种：英文

外文关键词：Quercetin; radiation-induced bystander-effect; HMGB1; ROS; Apoptosis

摘要：To investigate the protective effect of Quercetin (Que) on lung epithelial cells (BEAS-2B) induced bystander effect (RIBE) after heavy ion irradiation of A549 cells. A549 cells were irradiated with 2 Gy X heavy ion rays to obtain a conditioned medium. BEAS-2B was incubated with a conditioned medium or Que. CCK-8 assay was used to screen the optimal effective concentration of Que and detect cell proliferation. Cell number was measured by cell counter and apoptosis rate was measured by flow cytometry. HMGB1 and ROS levels were measured by ELISA. Western blot was used to detect the protein expression of HMGB1, TLR4, p65, Bcl-2, Bax, Caspase3 and Cleaved Caspase3. The growth and proliferation rate of BEAS-2B decreased while the apoptosis rate increased after conditioned medium stimulation, and Que intervention inhibited this effect. The expression of HMGB1 and ROS increased after conditioned medium stimulation, and this effect was inhibited by Que intervention. In addition, the conditioned medium increased the levels of proteins of HMGB1, TLR4, p65, Bax, Caspase3 and Cleaved Caspase 3, and decreased levels of Bcl-2 protein, but Que intervention de-creased the levels of HMGB1, TLR4, p65, Bax, Caspase3 and Cleaved Caspase 3proteins, and increased levels of Bcl-2 protein. The RIBE of BEAS-2B induced by irradiation of A549 is associated with HMGB1TLR4/ NF-& kappa;B signaling pathway in conditioned medium inducing apoptosis by activating ROS, and Que may block RIBE-induced apoptosis by regulating HMGB1/TLR4/NF-& kappa;B pathway.