文献类型：期刊文献

中文题名：扶正消癥方对小鼠H22移植性肝癌的生长抑制及VEGF、TNF-α表达的影响

英文题名：Effects of"Fuzheng Xiaozheng Decoction"on the Growth of Implanted Hepatocarcinoma and VEGF and TNF-α in H22 Mice

作者：周语平[1];韩维斌[1];刘光炜[1];张敏[1];万军鸽[1];刘辉[1];李华[1]

第一作者：周语平

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2009

卷号：43

期号：9

起止页码：64

中文期刊名：上海中医药杂志

外文期刊名：Shanghai Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：甘肃省中青年科技资金资助项目(3YS051-A25-025)

语种：中文

中文关键词：扶正消癥方;肝癌;血管内皮生长因子;肿瘤坏死因子-α

外文关键词：＂Fuzheng Xiaozheng Decoction＂; hepatocarcinoma; vascular endothelial growth factor; tumor necrosis factor-α

摘要：目的研究扶正消癥方对肝癌H22荷瘤小鼠的作用。方法以荷肝癌(H22)小鼠为模型,观察扶正消癥方的抗肿瘤作用,分别计算出抑瘤率、胸腺指数和脾脏指数,并用放免法和酶联免疫吸附实验分别测定血清中TNT-α和VEGF的含量。结果相当于生药3.5、7.1、14.2g/kg的扶正消癥方对肝癌(H22)小鼠的抑瘤率分别为15.95%、32.76%和39.22%。复方低剂量组、中剂量组和高剂量组与模型组的小鼠胸腺指数和脾脏指数比较,差异有显著性意义(P<0.01)。复方低剂量组、中剂量组和高剂量组与模型组对比血清中VEGF和TNF-α的含量差异有显著性意义(P<0.01)。结论扶正消癥方对肝癌H22有明显作用,具有提高机体免疫力、诱导肿瘤细胞坏死和抑制肿瘤浸润的的多重效应,具有进一步研究和开发的实验和临床应用价值。
Objective To study the effects of＂Fuzheng Xiaozheng Decoction＂on implanted hepatocarcinoma in H22 mice.Methods Implanted hepatocarcinoma in H22 mice was treated with＂Fuzheng Xiaozheng Decoction＂.The tumor-suppressing rate,thymus and spleen indexes were calculated;the contents of VEGF and TNF-αwere detected by radioimmunoassay and enzyme-linked immunosorbent assay.Results The tumor-supressing rales of 3.5,7.1 and 14.2 g/kg of＂Fuzheng Xiaozheng Decoction＂were respectively 15.95%,32.76%and 39.22%;there were great differences in thymus and spleen indexes and the contents of VEGF and TNF-αbetween treatment groups and model group（P〈0.01）.Conclusion＂Fuzheng Xiaozheng Decoction＂is effective in the treatment of H22 hepatocarcinoma to enhance immunity,induce tumor cell necrosis and inhibit tumor infiltration.