文献类型：期刊文献

中文题名：当归贝母苦参丸含药血清对胃癌细胞SGC-7901抑制作用机制的研究

英文题名：Effect of Danggui Beimu Kushen Wan Medicated Serum on Inhibition of Gastric Cancer Cells SGC-7901

作者：吴红彦[1];荣倩倩[1];李海龙[1];马春林[1];师金凤[1];陈杰[1]

第一作者：吴红彦

机构：[1]甘肃中医学院,甘肃省中医方药挖掘与创新转化重点实验室,甘肃省中药新产品创制工程实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2016

卷号：22

期号：2

起止页码：137

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：甘肃省高等学校2013年科研项目(2013A-088)

语种：中文

中文关键词：当归贝母苦参丸含药血清;胃癌;SGC-7901;环氧合酶-2;Bcl-2相关X蛋白;人第10号染色体缺失的磷酸酶

外文关键词：Danggui Beimu Kushen Wan medicated serum; gastric cancer; SGC-7901; cyclooxygenase-2; Bcl-associated X protein; phosphatase and tensin homolog deleted on chromosome ten;

摘要：目的:研究不同浓度当归贝母苦参丸含药血清抑制胃癌细胞SGC-7901的机制。方法:将120只Wistar大鼠分为当归贝母苦参丸高、中、低剂量组(0.3,0.2,0.1 g·kg^(-1))和正常组,每组30只,ig 2次/d,连续给药7 d,制备含药血清,以空白血清处理胃癌细胞SGC-7901为空白组,实验组以当归贝母苦参丸含药血清高、中、低剂量组处理SGC-7901细胞后用RTq PCR技术检测环氧合酶-2(COX-2),Bcl-2相关X蛋白(Bax),人第10号染色体缺失的磷酸酶(PTEN)mRNA表达水平、用免疫细胞化学技术检测COX-2,BAX,PTEN蛋白表达情况。结果:与正常组比较,高剂量组使SGC-7901细胞COX-2 mRNA的表达量下降68%,BAX和PTEN mRNA的表达量分别升高99.7%和85.6%(P<0.05),含药血清可以降低SGC-7901细胞COX-2的阳性表达率,升高其平均灰度值,升高BAX和PTEN的阳性表达率,减低其平均灰度值(P<0.05)。结论:当归贝母苦参丸含药血清能抑制胃癌细胞SGC-7901,BAX,PTEN,COX-2 mRNA和蛋白的表达。
Objective： To study the effect of Danggui Beimu Kushen Wan medicated serum on the inhibition of gastric cancer cells SGC-7901 and its antineoplastic mechanisms. Method： The 120 Wistar rats were divided into Danggui Beimu Kushen Wan high dose group,middle dose group and low dose group（0. 3,0. 2,0. 1 g·kg^-1） and normal group,n = 30 in each group,ig 2 times/d,continuous for 7 days to prepare drug serum.The rats whose gastric cancer cells SGC-7901 were treated by blank serum were used as blank group. In the experiment groups,SGC-7901 cells were treated by high dose,middle dose and low dose Danggui Beimu Kushen Wan medicated serum,and then RT-q PCR technique was used to detect the expression level of mRNA of cyclooxygenase-2（COX-2）,Bcl-associated X protein（Bax）,and phosphatase and tensin homolog deleted on chromosome ten（PTEN）. Immunocytochemical technique was used to test the protein expression level of COX-2,Bax,and PTEN. Result： Compared with the normal group,high dose group decreased the expression of COX-2mRNA in SGC-7901 cell by 68%,and increased the expression of Bax and PTEN mRNA by 99. 7% and 85. 6%respectively（P〈 0. 05）. Drug serum can reduce the positive expression rate of COX-2 for SGC-7901 cells,increase its average gray value. increase the positive expression rate of Bax and PTEN,rate and reduce the average gray value（P〈 0. 05）. Conclusion： Danggui Beimu Kushen Wan medicated serum could inhibit the expressions of Bax,PTEN and COX-2 at mRNA and protein level in SGC-7901 gastric cancer cells.