文献类型：期刊文献

中文题名：p38 MAPK在腹泻型肠易激综合征大鼠中的变化及其免疫调控作用

英文题名：Immunoregulatory effects of the p38 MAPK-signaling pathway in rats with diarrhea-predominant irritable bowel syndrome

作者：郭军雄[1];汪斌[1];马丽[1];康万荣[2];许小敏[1];徐生刚[1]

第一作者：郭军雄

机构：[1]河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖734000;[2]甘肃中医药大学,兰州730000

第一机构：河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖734000

年份：2019

卷号：27

期号：6

起止页码：709

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(81660759)~~

语种：中文

中文关键词：肠易激综合征;腹泻型;p38 MAPK信号通路;白细胞介素1β;白细胞介素6;肿瘤坏死因子α;大鼠

外文关键词：irritable bowel syndrome;diarrhea-predominant;p38 MAPK signaling pathway;IL-1β;IL-6;TNF-α;rats

摘要：目的探讨p38 MAPK信号在腹泻型肠易激综合征大鼠中的免疫调控作用。方法将40只SPF级Wistar大鼠(雌雄各半),随机分为D-IBS模型Ⅰ(造模7 d)组、Ⅱ(造模14 d)、III(造模21 d)组及空白对照组,每组10只。模型组采用慢性束缚联合番泻叶灌胃法建模,空白对照组灌服同等体积的纯净水。D-IBS大鼠分别于建模第7天、第14天和第21天剖杀,空白对照组于第21天剖杀。心脏采血,用酶联免疫吸附测定法(ELISA)检测各组大鼠血清IL-1β、IL-6和TNF-α含量;剖取结肠组织,HE染色观察大鼠结肠组织形态学,免疫组化法测大鼠结肠组织中p38 MAPK的蛋白表达。结果与空白对照组比较,模型组大鼠血清IL-1β、IL-6和TNF-α含量和结肠p38 MAPK蛋白表达阳性率增高(P<0.05,P<0.01),且p38 MAPK的表达与IL-1β、IL-6和TNF-α的水平呈正相关。结论慢性束缚联合番泻叶灌胃法复建的D-IBS大鼠模型,可能存在通过激活p38 MAPK信号通路,可上调p38 MAPK的表达,促进IL-1β、IL-6和TNF-α的释放,诱发肠黏膜低度炎症反应。
Objective To investigate the immunoregulatory effect of P38 mitogen activated protease(p38 MAPK)signals in rats with diarrhea-predominant irritable bowel syndrome(D-IBS).Methods Forty SPF Wistar rats(half male and half female)were randomly divided into a control group,and D-IBS model I(7 d),II(14 d)and III(21 d)groups,with 10 rats in each group.Rats in the model groups were developed through chronic restraint and senna decoction gavage,whereas the control rats were gavaged with the same volume of pure water.The D-IBS rates were killed on the seventh,fourteenth and twenty-first days,respectively.Rats in the control group were killed on the twenty-first day.The serum levels of IL-1β,IL-6 and TNF-αwere measured by ELISA.The pathological changes in colon tissue were observed with hematoxylin and eosin(H&E)staining,and the p38 MAPK protein expression in the colon tissue was assessed with immunohistochemistry.Results Compared with the control group,the IL-1β,IL-6 and TNF-αlevels in serum,and the p38 MAPK protein expression in colon tissue of the model groups were significantly higher(P<0.05,P<0.01).The expression of p38 MAPK was positively correlated with IL-1β,IL-6 and TNF-α.Conclusions The D-IBS rat model,developed through chronic restraint and senna decoction gavage,may have upregulation of the expression of p38 MAPK,promoting the release of IL-1β,IL-6 and TNF-α,and inducing mild inflammation of the intestinal mucosa by activating the p38 MAPK signal pathway.