文献类型：期刊文献

英文题名：Astragalus Polysaccharide Inhibits Ionizing Radiation-Induced Bystander Effects by Regulating MAPK/NF-κB Signaling Pathway in Bone Mesenchymal Stem Cells (BMSCs)

作者：Zhang, Liying[1];Luo, Yali[1];Lu, Zhiwei[1];He, Jinpeng[2,3];Wang, Lei[1];Zhang, Lixin[1];Zhang, Yiming[1];Liu, Yongqi[1,4]

第一作者：张利英

通信作者：Liu, YQ[1];Liu, YQ[2]

机构：[1]Gansu Univ Tradit Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou, Gansu, Peoples R China;[2]Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Gansu, Peoples R China;[3]Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China;[4]Gansu Univ Tradit Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Tradit Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2018

卷号：24

起止页码：4649

外文期刊名：MEDICAL SCIENCE MONITOR

收录：;Scopus(收录号：2-s2.0-85049746509);WOS:【SCI-EXPANDED(收录号:WOS:000437912700001)】；

基金：This study was supported by a grant from the National Natural Science Foundation of China [grant number 81473457]

语种：英文

外文关键词：Bystander Effect; Mesenchymal Stromal Cells; Polysaccharides

摘要：Background: This study investigated the effect of Astragalus polysaccharides (APS) on radiation-induced bystander effects (RIBE) in human bone mesenchymal stem cells (BMSCs) induced by irradiated A549 cells. Material/Methods: A549 cells were irradiated with 2 Gy X-rays to obtain conditioned medium. BMSCs were incubated with the conditioned medium or APS. The levels of reactive oxygen species (ROS) and TGF-beta were detected by ELISA. Cell survival, genomic instability, and DNA damages were detected by CCK-8 assay, colony formation assay, the micronucleus test and immunofluorescence assay, respectively. The protein and phosphorylation protein expression of p38, c-Jun N-terminal kinase (JNK), extracellular regulated protein kinase (ERK1/2), P65, and cyclooxygenase-2 (COX-2) in bystander effect cells were detected by Western blot. Results: The expression of COX-2 and ROS increased following stimulation with conditioned medium; this effect was inhibited by pre-exposing the cells to APS. BMSCs growth and colony formation rate decreased following stimulation with conditioned medium; this effect was suppressed by pre-exposing the cells to APS. In addition, the micronucleus rate and 53BP1 foci number increased after treatment with conditioned medium; this increase in BMSCs was inhibited by APS. The levels of phosphorylated p38, JNK, ERK1/2, NE-kappa B P65, and COX-2 proteins were increased by conditioned medium but were decreased by pre-treatment with APS. Conclusions: RIBE in BMSCs induced by the irradiated A549 was mediated by the ROS in the conditioned medium and might be related to MAPK/NF-kappa B signal pathways in BMSCs. APS may block RIBE through regulating the MAPK/NF-kappa B pathway.