文献类型：期刊文献

中文题名：红芪多糖对早期糖尿病肾病db/db小鼠肾脏保护作用及PKC/TIMP-1表达的影响

英文题名：Protection effect of Hedysarum polybotrys polysacchcaide on early diabetic nephropathy db/db mice and its influence on expression PKC/TIMP-1 in renal tissue

作者：祁雪艳[1];金智生[1];陈长浩[1];关雁[1];王倩[1];余海艳[1];张梅菊[1]

第一作者：祁雪艳

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：35

期号：18

起止页码：5053

中文期刊名：中国老年学杂志

外文期刊名：Chinese Journal of Gerontology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金地区科学基金项目资助(No.81160427)

语种：中文

中文关键词：红芪多糖;糖尿病肾病;蛋白激酶C(PKC);金属蛋白酶组织抑制剂(TIMP)-1;db/db小鼠

外文关键词：HPS;;Diabetic nephropathy;;PKC/TIMP-1;;db/db mice

摘要：目的通过研究红芪多糖(HPS)对糖尿病肾病(DN)db/db小鼠肾组织中PKC与金属蛋白酶组织抑制剂(TIMP)-1表达的影响,探讨HPS对DN小鼠肾脏的保护机制。方法将50只5周龄的雄性db/db小鼠随机分为5组:HPS高、中和低剂量组,替米沙坦阳性对照组,模型对照组;另设正常组,为10只同周龄的db/m小鼠。给药8 w,于给药前及给药后第2、4、6、8周末检测小鼠血糖浓度,第8周末收集小鼠24 h尿,检测24 h尿蛋白排泄量后处死小鼠,眼球取血并分离血清检测甘油三酯(TC)、总胆固醇(TG)等,并采用反转录聚合酶链式反应(RT-PCR)、免疫蛋白印迹检测肾组织PKC蛋白及IIMP-1 mRNA的表达。结果 HPS治疗8 w后,db/db小鼠的血糖、体重均较模型组降低,但只有高、中剂量组有统计学差异(P<0.05),且中剂量组下降明显(P<0.01);24 h蛋白尿排泄量与模型组相比均明显降低(P<0.05);与模型组比较各治疗组TC、TG均降低(P<0.05),其中中剂量组下降更明显(P<0.01)。与模型组比较,TIMP-1 mRNA的表达量除HPS低剂量组无改变外,其余治疗组均增强(P<0.05),且HPS中剂量组变化明显(P<0.01);PKC蛋白的表达在HPS高、中剂量组有所下降(P<0.05),且HPS中剂量组下降明显(P<0.01)。结论 HPS可能通过抑制肾小球系膜细胞上PKC蛋白的表达及增强TIMP-1mRNA的表达,减缓糖尿病肾病的病程进展。
Objective To study the influence of HPS on diabetic nephropathy( DN) db/db mice of activating the PKC and TIMP-1.Methods 50 db / db male mice( 5 weeks old) were randomly divided into HPS( low-dose,mid-dose,hing-dose groups),enalaprilat,model groups; 10 db / m mice( 5 weeks old) were employed as normal group. The level of blood glucose was measured at before and after intervention 2,4,6,8 weekends. The levels of blood urea nitrogen( BUN),serum creatinine( Scr),and 24 h urine protein were measured after 8 w,and the changes of mRNA and protein expressions of renal tissue PKC and TIMP-1 were detected by RT-PCR and Western blot. Results After interventing 8 w,compared with those of model group,the levels of blood glucose,body weight were decreased,but only the high,middle dose groups was statistically difference( P<0. 05),especially middle dose group was decreased significantly( P<0. 01); 24 h urine protein,the expressions of P38 MAPK and P38 MAPK,MMPs mRNA and the corresponding protein were all decreased( P <0. 05),except HPS low-dose group and enalaprilat group( P>0. 05),and in mid-dose group of HPS they were improved more significantly( P<0. 01). Conclusions HPS could delay the development of DN by inhibiting the P38 MAPK,MMPs protein and mRNA expressions in the glomerular mesangial cell membrane.