文献类型：期刊文献

英文题名：Proangiogenic potential of plasma exosomes from prostate cancer patients

作者：Wang, Chao[1];Zhou, Chuan[1];Wang, Dong[2];Zhang, Yun-Feng[2];Lv, Hao-Xuan[1];He, Han[1];Ren, Yong-qi[2];Wang, Jia[2];Zhou, Feng-Hai[1,3]

第一作者：Wang, Chao

通信作者：Zhou, FH[1];Zhou, FH[2]

机构：[1]Lanzhou Univ, Clin Med Coll 1, Lanzhou 73000, Peoples R China;[2]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[3]Gansu Prov Hosp, Dept Urol, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 1, Lanzhou 73000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou 73000, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Dept Urol, Lanzhou 730000, Peoples R China.

年份：2024

卷号：124

外文期刊名：CELLULAR SIGNALLING

收录：;Scopus(收录号：2-s2.0-85203649901);WOS:【SCI-EXPANDED(收录号:WOS:001316069900001)】；

基金：The Natural Science Foundation of Gansu Province (No.22JR5RA650). Research Fund Project of Internal Medicine Department, Gansu Provincial Hospital (22GSSYD-15). Scientific Research Foundation of Gansu Provincial People's Hospital (23GSSYD-12).r (No.22JR5RA650) . Research Fund Project of Internal Medicine Department, Gansu Provincial Hospital (22GSSYD-15) . Scientific Research Foundation of Gansu Provincial People's Hospital (23GSSYD-12) .

语种：英文

外文关键词：Prostate cancer; Exosomes; Angiogenesis; Hsa-miR-184

摘要：Angiogenesis plays a pivotal role in the progression and metastasis of solid cancers, including prostate cancer (PCa). While small extracellular vesicles derived from PCa cell lines induce a proangiogenic phenotype in vascular endothelial cells, the contribution of plasma exosomes from patients with PCa to this process remains unclear. Here, we successfully extracted and characterized plasma exosomes. Notably, a ring of PKH67-labeled exosomes was observed around the HUVEC nucleus using fluorescence microscopy, indicating the uptake of exosomes by HUVEC. At the cellular level, PCa plasma exosomes enhanced angiogenesis, proliferation, invasion, and migration of HUVEC cells. Moreover, PCa plasma exosomes promoted angiogenesis and aortic sprouting. MicroRNAs are the most common genetic material in exosomes, and to identify miRNAs associated with the angiogenic response, we performed small RNA sequencing followed by RT-qPCR and bioinformatics analysis. These analyses revealed distinct miRNA profiles in plasma exosomes from patients with PCa compared to healthy individuals. Notably, hsa-miR-184 emerged as a potential regulator implicated in the proangiogenic effects of PCa plasma exosomes.