文献类型：期刊文献

英文题名：1-Hydroxy-3,7,8-Trimethoxyxanthone Suppresses the Malignant Proliferation of Human Bone Marrow Mesenchymal Stem Cells in Colon Cancer Microenvironment

作者：Zhao, Hui-qiao[1];Lu, Nian-hua[2];Zhang, Xu-dong[1];Liu, Na[1];Jing, Ming[1]

第一作者：Zhao, Hui-qiao

通信作者：Jing, M[1]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Hebei North Univ, Coll Tradit Chinese Med, Zhangjiakou 075061, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2019

卷号：15

期号：2

起止页码：156

外文期刊名：INTERNATIONAL JOURNAL OF PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000462668200001)】；

基金：The authors are grateful to Prof. Shi Yanbin and Dr. Xu Zhen of Lanzhou University and Dr. Chen Guangxin of Shanxi University for providing language help. This work was supported by the National Natural Science Foundation of China [grant numbers: 81560717].

语种：英文

外文关键词：1-hydroxy-3,7,8-trimethoxyxanthone (HTX); malignant proliferation; human bone marrow mesenchymal stem cells (HMSCs); colon cancer

摘要：Background and Objective: Human bone marrow mesenchymal stem cells (HMSCs) have been widely used to study tumor gene therapy. However, they may be induced malignant proliferation in some cancers. Therefore, how to decrease the side effects of HMSCs has been becoming a hot topic. In this study, the role of 1-hydroxy-3,7,8-trimethoxyxanthone (HTX) on HMSCs in colon cancer microenvironment in vitro was evaluated. Materials and Methods: Non-contact co-culture system of colon cancer cells SW480 and HMSCs was established using Transwell cell culture chambers. 20, 10, 5 mu g mL(-1) of HTX was used to treat HMSCs in the colon cancer microenvironment. The proliferation of HMSCs in each group was tested using the MTT assay. The activity of HMSCs in each group was tested using Transwell invasion and migration assays. RT-PCR assay, Western Blot assay and ELISA assay were used to detect the expression of related genes and proteins. Results: In colon cancer microenvironment, the proliferation, invasion and migration of HMSCs significantly increased, however, supplemented with HTX isolated from Tibetan medicine Gentianopsis paludosa significantly improved the side effects. Furthermore, HTX inhibited the expression of IL-6 and the phosphorylation of STAT3 signalling pathway in HMSCs which was reduced by colon cancer microenvironment. Conclusion: All the results indicated that HTX inhibited the malignant proliferation of HMSCs in colon cancer microenvironment by inhibiting IL-6/STAT3 signaling pathway. The HTX may be a novel chemical inhibitor for gene therapy of colon cancer with HMSCs as carrier.